Dataset on the compounds from the leaves of Vietnamese Machilus thunbergii and their anti-inflammatory activity

Dao Cuong To; Le Minh Hoang; Hoa Thi Nguyen; Truong Thi Viet Hoa; Nhung Truong Thi Thuy; Manh Hung Tran; Phi Hung Nguyen; Phuong Dai Nguyen Nguyen; Ngu Truong Nhan; Nguyen Thi Thu Tram

doi:10.1016/j.dib.2023.109713

Dataset on the compounds from the leaves of Vietnamese Machilus thunbergii and their anti-inflammatory activity

Data Brief. 2023 Oct 22:51:109713. doi: 10.1016/j.dib.2023.109713. eCollection 2023 Dec.

Affiliations

¹ Phenikaa University Nano Institute (PHENA), Phenikaa University, Yen Nghia, Ha Dong, Hanoi 12116, Vietnam.
² School of Medicine & Pharmacy, The University of Danang, Hoa Quy, Ngu Hanh Son, Da Nang City 550000, Vietnam.
³ Institute of Natural Products Chemistry, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Cau Giay, Hanoi 122100, Vietnam.
⁴ Tay Nguyen University, 567 Le Duan, Buon Ma Thuot, Dak Lak 630000, Vietnam.
⁵ Faculty of Basic Sciences, Can Tho University of Medicine and Pharmacy, 179 Nguyen Van Cu, An Khanh Ward, Ninh Kieu District, Can Tho 94000, Vietnam.

Abstract

Machilus thunbergii has a history of traditional applications including treating dyspepsia, apoplexy, headaches, abdominal pain, abdominal distension, and leg edema [1]. It is also employed for alleviating allergies, inflammation, pain relief, promoting blood circulation, addressing costal chondritis, and sinusitis [2]. Research into the chemical composition of M. thunbergii has revealed the presence of lignans, flavonoids, lactones, and essential oils [1,[3], [4], [5]. While some investigations have explored the inhibitory effects of extracts and lignan compounds from this species on NO production [6], [7], [8], there has been no research into the flavonoids isolated from this plant and their potential for inhibiting NO production, given our reachable referencing. The ethyl acetate (EtOAc) soluble fraction of M. thunbergii leaves was subjected to column chromatography (CC) using silica gel and Sephadex LH-20 for compound isolation. Nuclear magnetic resonance (NMR) data primarily facilitated the determination of isolated compound structures. Anti-inflammatory activity was evaluated against lipopolysaccharide (LPS)-induced nitric oxide (NO) production in macrophage RAW264.7 cells. Anti-inflammatory activity-guided fractionation led to the isolation of twelve secondary metabolites (1-12). The compounds were identified as quercetin (1), kaempferol (2), rhamnetin (3), quercitrin (4), hyperoside (5), reynoutrin (6), guaijaverin (7), afzelin (8), astragalin (9), rutin (10), kaempferol-3-O-rutinoside (11), and rhamnetin-3-O-rutinoside (12). Compounds 3, 5, 6, 9, 11, and 12 were isolated from M. thunbergii for the first time. Evaluation against LPS-induced NO production in macrophage RAW264.7 cells showed that 1-3 exhibited potent inhibitory activity with IC₅₀ values of 15.45, 25.44, and 19.82 µM, respectively. Compounds 4-9 demonstrated IC₅₀ values ranging from 42.15 to 67.42 µM, while 10-12 exhibited inactivity (IC₅₀ > 100 µM).

Keywords: Cytotoxic; Flavonoid; Inflammation; NO production; RAW264.7 cells.