Development of a touchdown droplet digital PCR assay for the detection and quantitation of human papillomavirus 16 and 18 from self-collected anal samples

Gholamreza Haqshenas; Suzanne M Garland; Prisha Balgovind; Alyssa Cornall; Jennifer Danielewski; Monica Molano; Dorothy A Machalek; Gerald Murray

doi:10.1128/spectrum.01836-23

Development of a touchdown droplet digital PCR assay for the detection and quantitation of human papillomavirus 16 and 18 from self-collected anal samples

Microbiol Spectr. 2023 Dec 12;11(6):e0183623. doi: 10.1128/spectrum.01836-23. Epub 2023 Nov 14.

Authors

Gholamreza Haqshenas^{1

2

3}, Suzanne M Garland^{1

2

3}, Prisha Balgovind^{2

3}, Alyssa Cornall^{1

2

3}, Jennifer Danielewski^{2

3}, Monica Molano², Dorothy A Machalek^{2

3

4}, Gerald Murray^{1

2

3}

Affiliations

¹ Department of Obstetrics and Gynaecology, University of Melbourne , Melbourne, Victoria, Australia.
² Centre for Women's Infectious Diseases, The Royal Women's Hospital , Melbourne, Victoria, Australia.
³ Murdoch Children's Research Institute , Melbourne, Victoria, Australia.
⁴ The Kirby Institute, UNSW Sydney , Sydney, New South Wales, Australia.

Abstract

The quantity of the human papillomavirus (HPV) is associated with disease outcome. We designed an accurate and precise digital PCR assay for quantitating HPV in anal samples, a sample type that is typically problematic due to the presence of PCR inhibitors.

Keywords: anal samples; droplet digital PCR; human papillomavirus.

MeSH terms

Anal Canal
Human papillomavirus 16* / genetics
Humans
Papillomaviridae / genetics
Papillomavirus Infections* / diagnosis
Polymerase Chain Reaction

Grants and funding

APP1197951/DHAC | National Health and Medical Research Council (NHMRC)