OsCIPK9 Interacts with OsSOS3 and Affects Salt-Related Transport to Improve Salt Tolerance

Zhenling Zhou; Weijie Tang; Zhiguang Sun; Jingfang Li; Bo Yang; Yan Liu; Baoxiang Wang; Dayong Xu; Jianchang Yang; Yunhui Zhang

doi:10.3390/plants12213723

OsCIPK9 Interacts with OsSOS3 and Affects Salt-Related Transport to Improve Salt Tolerance

Plants (Basel). 2023 Oct 30;12(21):3723. doi: 10.3390/plants12213723.

Authors

Zhenling Zhou^{1

2}, Weijie Tang^{3

4}, Zhiguang Sun¹, Jingfang Li¹, Bo Yang¹, Yan Liu¹, Baoxiang Wang¹, Dayong Xu¹, Jianchang Yang², Yunhui Zhang^{3

4

5}

Affiliations

¹ Lianyungang Academy of Agricultural Sciences, Lianyungang 222000, China.
² Jiangsu Key Laboratory of Crop Genetics and Physiology, Jiangsu Key Laboratory of Crop Cultivation and Physiology, Agricultural College of Yangzhou University, Yangzhou 225009, China.
³ Provincial Key Laboratory of Agrobiology, Institute of Germplasm Resources and Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
⁴ Zhongshan Biological Breeding Laboratory, No. 50 Zhongling Street, Nanjing 210014, China.
⁵ Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, China.

Abstract

Salt is harmful to crop production. Therefore, it is important to understand the mechanism of salt tolerance in rice. CIPK genes have various functions, including regulating salt tolerance and other types of stress and nitrogen use efficiency. In rice, OsCIPK24 is known to regulate salt tolerance, but other OsCIPKs could also function in salt tolerance. In this study, we identified another OsCIPK-OsCIPK9-that can regulate salt tolerance. Knockout of OsCIPK9 in rice could improve salt tolerance. Through expression analyses, OsCIPK9 was found to be mainly expressed in the roots and less expressed in mature leaves. Meanwhile, OsCIPK9 had the highest expression 6 h after salt treatment. In addition, we proved the interaction between OsCIPK9 and OsSOS3. The RNA-seq data showed that OsCIPK9 strongly responded to salt treatment, and the transporters related to salt tolerance may be downstream genes of OsCIPK9. Finally, haplotype analyses revealed that Hap6 and Hap8 mainly exist in indica, potentially providing a higher salt tolerance. Overall, a negative regulator of salt tolerance, OsCIPK9, which interacted with OsSOS3 similarly to OsCIPK24 and influenced salt-related transporters, was identified, and editing OsCIPK9 potentially could be helpful for breeding salt-tolerant rice.

Keywords: OsCIPK9; OsSOS3; rice; salt tolerance; sat-related transporters.

Grants and funding

Lianyungang “521 Project” scientific research project, The Financial Grant Support Program of Lianyungang City (QNJJ1803; QNJJ2201), Special Project of Science and Technology in Northern Jiangsu Province (LYG-SZ201930), The Natural Science Foundation of Jiangsu Province (BK20221276) supported this study. The funding agencies had no role in the study design, data collection and analysis, decision to publish or manuscript preparation.