CRISPR/Cas12-based electrochemical biosensors for clinical diagnostic and food monitoring

Clianta Yudin Kharismasari; Irkham; Muhammad Ihda H L Zein; Ari Hardianto; Salma Nur Zakiyyah; Abdullahi Umar Ibrahim; Mehmet Ozsoz; Yeni Wahyuni Hartati

doi:10.1016/j.bioelechem.2023.108600

CRISPR/Cas12-based electrochemical biosensors for clinical diagnostic and food monitoring

Bioelectrochemistry. 2024 Feb:155:108600. doi: 10.1016/j.bioelechem.2023.108600. Epub 2023 Nov 5.

Authors

Clianta Yudin Kharismasari¹, Irkham¹, Muhammad Ihda H L Zein¹, Ari Hardianto¹, Salma Nur Zakiyyah¹, Abdullahi Umar Ibrahim², Mehmet Ozsoz³, Yeni Wahyuni Hartati⁴

Affiliations

¹ Department of Chemistry, Faculty of Mathematics and Natural Sciences, Padjajaran University, Sumedang 45363, Indonesia.
² Department of Biomedical Engineering, Near East University, Mersin 99138, Turkey; Operational Research Centre in Healthcare, Near East University, Mersin 10, TRNC, Turkey.
³ Department of Biomedical Engineering, Near East University, Mersin 99138, Turkey.
⁴ Department of Chemistry, Faculty of Mathematics and Natural Sciences, Padjajaran University, Sumedang 45363, Indonesia. Electronic address: yeni.w.hartati@unpad.ac.id.

PMID: 37956622
DOI: 10.1016/j.bioelechem.2023.108600

Abstract

Each organism has a unique sequence of nitrogenous bases in in the form of DNA or RNA which distinguish them from other organisms. This characteristic makes nucleic acid-based detection extremely selective and compare to other molecular techniques. In recent years, several nucleic acid-based detection technology methods have been developed, one of which is the electrochemical biosensor. Electrochemical biosensors are known to have high sensitivity and accuracy. In addition, the ease of miniaturization of this electrochemical technique has garnered interest from many researchers. On the other hand, the CRISPR/Cas12 method has been widely used in detecting nucleic acids due to its highly selective nature. The CRISPR/Cas12 method is also reported to increase the sensitivity of electrochemical biosensors through the utilization of modified electrodes. The electrodes can be modified according to detection needs so that the biosensor's performance can be improved. This review discusses the application of CRISPR/Cas12-based electrochemical biosensors, as well as various electrode modifications that have been successfully used to improve the performance of these biosensors in the clinical and food monitoring fields.

Keywords: Biosensors; CRISPR; Cas12; Diagnostic; Electrochemistry; Food monitoring.

Publication types

Review

MeSH terms

Biosensing Techniques* / methods
CRISPR-Cas Systems / genetics
DNA / chemistry
DNA / genetics
Electrochemical Techniques
Nucleic Acids*

Substances

DNA
Nucleic Acids