A novel nanozyme (urea@Cu-NF) was synthesized by self-assembly of urea and copper phosphate with urea as plasticizer. Urea@Cu-NF exhibited excellent peroxidase-like activity with the ability to oxidize TMB in the presence of H2O2. However, its peroxidase-like activity could be inhibited by resveratrol, leading to an absorption decrease in the intensity of oxTMB. Based on this phenomenon, a colorimetric method was designed for resveratrol detection. The colorimetric reaction could be completed within 20 min with a linear range of 1-120 μM. The limit of detection (LOD) of resveratrol is 0.43 μM. Our experimental results demonstrate that urea@Cu-NF has enormous potential to function as a cheap and accurate quality detection tool.