Molecularly imprinted polymers-aptamer electrochemical sensor based on dual recognition strategy for high sensitivity detection of chloramphenicol

Lingjun Geng; Jiashuai Sun; Mengyue Liu; Jingcheng Huang; Jiwei Dong; Zhen Guo; Yemin Guo; Xia Sun

doi:10.1016/j.foodchem.2023.137933

Molecularly imprinted polymers-aptamer electrochemical sensor based on dual recognition strategy for high sensitivity detection of chloramphenicol

Food Chem. 2024 Mar 30;437(Pt 2):137933. doi: 10.1016/j.foodchem.2023.137933. Epub 2023 Nov 7.

Authors

Lingjun Geng¹, Jiashuai Sun¹, Mengyue Liu¹, Jingcheng Huang¹, Jiwei Dong¹, Zhen Guo¹, Yemin Guo², Xia Sun³

Affiliations

¹ School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, Shandong 255049, China; Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo, Shandong 255049, China; Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun Xilu, Zibo, Shandong 255049, China.
² School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, Shandong 255049, China; Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo, Shandong 255049, China; Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun Xilu, Zibo, Shandong 255049, China. Electronic address: gym@sdut.edu.cn.
³ School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, Shandong 255049, China; Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo, Shandong 255049, China; Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun Xilu, Zibo, Shandong 255049, China. Electronic address: sunxia2151@sina.com.

PMID: 37951077
DOI: 10.1016/j.foodchem.2023.137933

Abstract

In this paper, an electrochemical sensor based on a dual recognition strategy of molecularly imprinted polymers (MIPs) and aptamer (Apt) has been designed for the high sensitivity detection of chloramphenicol (CAP). Here, MIPs and Apt have provided dual recognition sites to greatly improve the specific recognition ability of the sensor. Chitosan-multi-walled carbon nanotubes (CS-MWNTs) and AuNPs (gold nanoparticles) have been used for their excellent electrical conductivity. When CAP existed in the detection environment, the imprinted cavities with specific recognition ability bound to CAP through forces such as hydrogen bonds. It hindered the rate of electron transfer and resulted in a decrease in current value. Quantitative detection of CAP could be achieved after analyzing the relationship between the concentration of CAP and the change of current value. After optimizing the experimental parameters, the detection range of the sensor was 10^-8 g/L-10^-2 g/L with the limit of detection of 3.3 × 10^-9 g/L, indicating that the sensor had a high practical application potential.

Keywords: Chitosan-multi-walled carbon nanotubes; Chloramphenicol; Dual recognition strategy; Electrochemical sensor; Gold nanoparticles.

MeSH terms

Chloramphenicol
Electrochemical Techniques / methods
Electrodes
Gold / chemistry
Limit of Detection
Metal Nanoparticles* / chemistry
Molecular Imprinting* / methods
Molecularly Imprinted Polymers
Nanotubes, Carbon* / chemistry
Polymers / chemistry

Substances

Chloramphenicol
Molecularly Imprinted Polymers
Gold
Nanotubes, Carbon
Polymers