Seasonal changes in diet, immune function, and oxidative stress in three passerines inhabiting a Mediterranean climate

Abstract

Oxidative status and immune function are energy-demanding traits closely linked to diet composition, particularly resource availability and nutritional value. In seasonal environments, nutrient availability and diet quality fluctuate, potentially influencing these traits. However, limited evidence exists regarding these dietary effects on immune function in seasonal environments. In this study, we employed stable isotope analysis to assess the impact of seasonal changes in niche width and trophic level (i.e., δ¹⁵N) on two immune variables (hemolysis and hemagglutination scores) and two oxidative status parameters (lipid peroxidation and total antioxidant capacity) in three passerine species: Zonotrichia capensis (omnivorous), Troglodytes aedon (insectivorous), and Spinus barbatus (granivorous). We found that hemolysis scores varied seasonally in Z. capensis, with higher values in winter compared to summer. Total antioxidant capacity (TAC) also increased during the winter in Z. capensis and S. barbatus. The isotopic niche width for Z. capensis and S. barbatus was smaller in winter than in summer, with the omnivorous species exhibiting a decrease in δ¹⁵N. Despite the seasonal shifts in ecological and physiological traits in Z. capensis, we identified no correlation between immune response and TAC with trophic level. In contrast, in the granivorous S. barbatus, the lower trophic level resulted in an increase in TAC without affecting immunity. Our findings revealed that dietary shifts do not uniformly impact oxidative status and immune function across bird species, highlighting species-specific responses to seasonal changes. This underscores the importance of integrating ecological and evolutionary perspectives when examining how diet shapes avian immunity and oxidative balance.

Keywords: Antioxidant; Birds; Diet; Immunity; Oxidative stress; Seasonality.