Effects of ultra-high pressure assisted extraction on the structure, antioxidant and hypolipidemic activities of Porphyra haitanensis polysaccharides

Mingjing Zheng; Xin Tian; Zhipeng Li; Tao Hong; Yanbing Zhu; Yuanfan Yang; Qingbiao Li; Hui Ni; Zedong Jiang

doi:10.1016/j.foodchem.2023.137856

Effects of ultra-high pressure assisted extraction on the structure, antioxidant and hypolipidemic activities of Porphyra haitanensis polysaccharides

Food Chem. 2024 Mar 30;437(Pt 2):137856. doi: 10.1016/j.foodchem.2023.137856. Epub 2023 Oct 29.

Authors

Mingjing Zheng¹, Xin Tian², Zhipeng Li¹, Tao Hong¹, Yanbing Zhu¹, Yuanfan Yang¹, Qingbiao Li¹, Hui Ni³, Zedong Jiang⁴

Affiliations

¹ College of Ocean Food and Biological Engineering, Jimei University, Xiamen, Fujian 361021, China; Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Xiamen, Fujian 361021, China.
² College of Ocean Food and Biological Engineering, Jimei University, Xiamen, Fujian 361021, China.
³ College of Ocean Food and Biological Engineering, Jimei University, Xiamen, Fujian 361021, China; Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Xiamen, Fujian 361021, China; Xiamen Ocean Vocational College, Xiamen 361021, Fujian, China.
⁴ College of Ocean Food and Biological Engineering, Jimei University, Xiamen, Fujian 361021, China; Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Xiamen, Fujian 361021, China. Electronic address: zdjiang@jmu.edu.cn.

PMID: 37948798
DOI: 10.1016/j.foodchem.2023.137856

Abstract

Porphyra haitanensis polysaccharides (PHPs) have potential antioxidant and hypolipidemic activities, but still need improvement. Therefore, ultra-high pressure (UHP) assisted extraction was applied to modify the structure, antioxidant, and hypolipidemic activities of PHPs. UHP assisted extraction increased the total sugar, uronic acid, and 3,6-anhydro-ʟ-galactose contents of PHP, which increased by 15.85 %-16.12 %, 18.95 %-24.32 %, 20.54 %-23.66 % with 500-600 MPa UHP, respectively. Meanwhile, UHP modified PHP became more rough and porous than native PHP. Besides, UHP assisted extracted PHP showed better in vitro antioxidant and hypolipidemic abilities. Especially, 500-600 MPa UHP increased 72.43 %-86.42 % 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacities, 12.32 %-12.82 % pancreatic lipase inhibitory ability, and 14.93 %-15.23 % glycocholate binding abilities of PHP (p < 0.05). Moreover, UHP assisted extracted PHP greatly decreased the lipid droplet and triglyceride contents of 3T3-L1 adipocytes (p < 0.05). Our findings can provide the theoretical basis for the high value utilization of Porphyra haitanensis and its polysaccharides with UHP modification.

Keywords: 3T3-L1 preadipocytes; Antioxidant activity; Hypolipidemic activity; Porphyra haitanensis polysaccharides; Ultra-high pressure.

MeSH terms

Antioxidants* / chemistry
Galactose / metabolism
Polysaccharides / chemistry
Porphyra* / chemistry

Substances

Antioxidants
Polysaccharides
Galactose