Failure to apply standard limit-of-detection or limit-of-quantitation criteria to specialized pro-resolving mediator analysis incorrectly characterizes their presence in biological samples
Nat Commun. 2023 Nov 9;14(1):7172.
doi: 10.1038/s41467-023-41766-w.
1 Systems Immunity Research Institute, School of Medicine, Cardiff University, CF14 4XN, Cardiff, Wales, UK. O-donnellvb@cardiff.ac.uk.
2 Chair of Food Chemistry, Faculty of Mathematics and Natural Sciences University of Wuppertal, Gausstraße 20, 42119, Wuppertal, Germany.
3 Division of Clinical Pharmacology, Vanderbilt University, 502A Robinson Research Building, Nashville, TN, 37232-6602, USA.
4 Medical Research Council Mitochondrial Biology Unit, University of Cambridge, Cambridge, CB2 0XY, UK.
5 School of Pharmacy and Pharmaceutical Sciences, Cardiff University, Cardiff, CF10 3AT, UK.
6 Institute of Pharmaceutical Chemistry, Goethe University Frankfurt, Max-von-Laue-Str. 9, 60438, Frankfurt, Germany.
7 Department of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, PA, 15261, USA.
8 Institute of Biochemistry, University Medicine Berlin - Charité, Berlin, Germany.
9 Department of Chemistry, University of Washington, Seattle, WA, 98195, USA.
10 Rheumatology Unit, Dep. of Medicine, Solna, Karolinska Institutet & Karolinska University Hospital, Stockholm, Sweden.
11 Department of Systems Pharmacology and Translational Therapeutics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA.
12 Department of Pharmacology, University of Colorado Denver, Aurora, CO, 80045, USA.
13 Department of Pharmacology, Vanderbilt University, Nashville, TN, 37232, USA.
14 Institute for Translational Medicine and Therapeutics, Smilow Center for Translational Research, University of Pennsylvania, Philadelphia, PA, 19104, USA. garret@upenn.edu.