Quantification of risperidone and paliperidone by liquid chromatography-tandem mass spectrometry in biological fluids and solid tissues obtained from two deceased using the standard addition method

Hideki Nozawa; Kayoko Minakata; Koutaro Hasegawa; Itaru Yamagishi; Naotomo Miyoshi; Kenta Yuyama; Masako Suzuki; Takuya Kitamoto; Minako Kondo; Osamu Suzuki

doi:10.1016/j.legalmed.2023.102340

Quantification of risperidone and paliperidone by liquid chromatography-tandem mass spectrometry in biological fluids and solid tissues obtained from two deceased using the standard addition method

Leg Med (Tokyo). 2023 Nov 5:69:102340. doi: 10.1016/j.legalmed.2023.102340. Online ahead of print.

Authors

Affiliations

¹ Department of Legal Medicine, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu 431-3192, Japan. Electronic address: hnozawa@hama-med.ac.jp.
² Department of Legal Medicine, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu 431-3192, Japan.
³ Advanced Research Facilities and Services, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu 431-3192, Japan.

PMID: 37945391
DOI: 10.1016/j.legalmed.2023.102340

Abstract

Risperidone (RIS) is an atypical antipsychotic agent and its 9-hydroxylated metabolite named paliperidone (PAL) also has pharmacological properties similar to that of RIS. Quantifications of RIS and PAL in authentic human biological fluids and solid tissues by liquid chromatography (LC)-tandem mass spectrometry (MS/MS) have not been reported yet although those in plasma (and blood) were reported abundantly. In the present work, a quantification method for RIS and PAL based on the standard addition method was devised and validated for the human fluid and solid tissue specimens. RIS and PAL in biological fluids were quantified only after their dilution and deproteinization. The concentrations of RIS and PAL in the heart whole blood, pericardial fluid, stomach contents, bile, urine, liver, kidney and cerebrum were determined for a deceased who had been treated with RIS therapeutically, and also a deceased who had ingested RIS with other drugs intentionally. To our knowledge, this is the first report on the quantification of RIS and PAL by LC-MS/MS in the authentic human tissues and biological fluids.

Keywords: Authentic human specimens; Liquid chromatography–tandem mass spectrometry; Paliperidone (9-hydroxy risperidone); Risperidone; Standard addition method.

Publication types

Case Reports