Combined pollution and soil microbial effect of pesticides and microplastics in greenhouse soil of suburban Tianjin, Northern China

Abstract

Current-used pesticides (CUPs) and plastic films are essential materials used in greenhouse cultivation, which can lead to the residual accumulation of CUPs and microplastics (MPs) over time. The impact of CUPs and MPs on soil quality and food safety cannot be overlooked. However, the combined pollution resulting from CUPs and MPs in greenhouse soil remains poorly understood. In this study, we conducted a survey at 30 greenhouse sites in the Wuqing District of Tianjin, China, to investigate the pollution levels and characteristics of CUPs and MPs using QuEChERS combined with LC-MS/MS, and density extraction, 30% H₂O₂ digestion and micro-fourier transform infrared spectroscopy, respectively. Additionally, we aimed to evaluate the interactions among these two pollutants, soil physicochemical properties, and the bacterial community in the soil. CUPs were frequently detected in the examined soil samples; however, they posed no significant ecological risks due to their low levels. Furthermore, MPs, which predominantly comprised fragmented and fibrous polyethylene (PE) and polypropylene (PP) particles smaller than 1.0 mm, could potentially degrade into nanoplastics, which might subsequently enter the food chain and pose a serious threat to human health. We observed no substantial correlations between CUPs and MPs, except for a negative correlation between dimethomorph and film MPs. The soil pH and total organic carbon (TOC) exhibited interactions with both types of pollutants, whereas soil clay content (CC) only correlated with CUPs, and soil available nitrogen (AN) only correlated with MPs. The variability of soil bacterial communities among the 30 sampling sites was minimal, with the dominant genus being Bacillus. Soil pH, TOC, and CC collectively exerted a strong influence on the microbial community across all samples; however, the effects of CUPs and MPs on the soil microbial structure were marginal. These results contribute to a comprehensive understanding of the environmental stress and ecological risks associated with the combined pollution of CUPs and MPs.

Keywords: Current-used pesticides; Ecological risk; Greenhouse soil; Microbial community; Microplastics.