Lead speciation in body tissues, gastrointestinal contents, and feces of lead-exposed wild rats (Rattus rattus)

Abstract

The toxic effects of lead (Pb) are an ongoing concern for which research continues to seek a solution. In Pb-contaminated areas, Pb concentrations in the environment and organisms are quantified to assess the degree of contamination. Understanding organismal uptake of Pb and its behavior in the body requires distinguishing Pb chemical species. We used Pb L₃-edge X-ray absorption near-edge structure (XANES) to study the distribution of Pb species in body tissues, digestive tract contents, and feces of wild rats (Rattus rattus) collected from a heavily Pb-contaminated mining area in Zambia. Freeze-drying improved the XANES spectrum quality by approximately 10 μg-Pb/g-dry through concentrating the Pb without changing its chemical state from its wet state. We successfully identified and analyzed the Pb species in seven different tissues (bone, muscle, liver, kidney, spleen, lung, and brain), three different digestive-tract contents (stomach, small intestine, and colon), and feces from three wild rats. We described chemical-form-based details of Pb uptake and distribution that are common among rats, such as the increased Pb binding with thiol groups through the digestive system, distribution of mobile Pb to hematopoietic organs and vascular-related tissues, and Pb binding to thiol groups, especially in the liver and brain.

Keywords: Body tissues; Gastrointestinal contents; Lead speciation; Wild rats; X-ray absorption near-edge structure.