Evaluation of the feasibility of using inorganic and organic acids for the extraction of amino acids from high-protein material (mealworm larvae) by chemical hydrolysis

Abstract

High-protein mealworm larvae (Tenebrio molitor) were treated with organic and inorganic acids in order to check the feasibility of applying acid hydrolyzates derived from them. Hydrolysis was optimized for the highest content of amino acids that have beneficial effects on plant development. The response surface method served the purpose of selecting optimal process conditions for such media as phosphoric acid, citric acid, acetic acid and mixtures of phosphoric and citric acids. The elemental composition of the best formulations and their amino acid profile was determined. Plant tests on cucumber (germination and pot tests) revealed the beneficial effect of the hydrolyzates on plant biometric parameters. By offering valuable insights into the relationship between fertilizer treatments and plant growth, our study contributes to developing sustainable agricultural practices and improved crop productivity.

Keywords: Bioavailability; Biostimulant; Hydrolyzate; amino acids; hydrolysis; mealworm larvae.