Synthesis of Polymeric Mimics of Ice-Binding Proteins

Christian C M Sproncken; Christophe Detrembleur; Ilja K Voets

doi:10.1007/978-1-0716-3503-2_14

Synthesis of Polymeric Mimics of Ice-Binding Proteins

Methods Mol Biol. 2024:2730:203-210. doi: 10.1007/978-1-0716-3503-2_14.

Authors

Christian C M Sproncken¹, Christophe Detrembleur², Ilja K Voets³

Affiliations

¹ Department of Chemical Engineering and Chemistry & Institute for Complex Molecular Systems, Eindhoven University of Technology, Eindhoven, the Netherlands.
² Center for Education and Research on Macromolecules (CERM) CESAM Research Unit, Department of Chemistry, University of Liège, Liège, Belgium.
³ Department of Chemical Engineering and Chemistry & Institute for Complex Molecular Systems, Eindhoven University of Technology, Eindhoven, the Netherlands. i.voets@tue.nl.

PMID: 37943460
DOI: 10.1007/978-1-0716-3503-2_14

Abstract

Cobalt-mediated radical polymerization (CMRP) enables the preparation of both short and long polymers from acrylic and vinyl ester monomers with low dispersity. Here we describe the synthesis, purification, and characterization of polymeric mimics of ice-binding proteins based on the water-soluble polymer poly(vinyl alcohol) by CMRP. Block copolymers of poly(vinyl alcohol) and poly(acrylic acid) were prepared from the precursor copolymers poly(vinyl acetate)-b-poly(acrylonitrile) upon hydrolysis. Copolymers comprising up to hundreds of monomers and dispersities M_w/M_n < 1.3 were produced by this method.

Keywords: 1H NMR spectroscopy; Cobalt-mediated radical polymerization; Controlled radical polymerization; Poly(vinyl alcohol); Poly(vinyl alcohol)-b-poly(acrylic acid); Polymeric antifreeze; Polymeric mimic of ice-binding proteins; Size exclusion chromatography.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acrylonitrile*
Carrier Proteins*
Cobalt
Ice
Polymers
Polyvinyl Alcohol

Substances

Carrier Proteins
Ice
Polyvinyl Alcohol
Polymers
Acrylonitrile
Cobalt