Measurement of Ice-Binding Protein Activity in Highly Alkaline Environments

Elizabeth A Delesky; Aparna J Lobo; Wil V Srubar 3rd

doi:10.1007/978-1-0716-3503-2_10

Measurement of Ice-Binding Protein Activity in Highly Alkaline Environments

Methods Mol Biol. 2024:2730:135-154. doi: 10.1007/978-1-0716-3503-2_10.

Authors

Elizabeth A Delesky¹, Aparna J Lobo², Wil V Srubar 3rd³

Affiliations

¹ Department of Civil, Environmental, and Architectural Engineering, University of Colorado Boulder, Boulder, CO, USA.
² Department of Chemical and Biological Engineering, University of Colorado Boulder, Boulder, CO, USA.
³ Department of Civil, Environmental, and Architectural Engineering, University of Colorado Boulder, Boulder, CO, USA. wsrubar@colorado.edu.

PMID: 37943456
DOI: 10.1007/978-1-0716-3503-2_10

Abstract

Ice-binding proteins (IBPs) exhibit a disruptive potential to prevent ice growth in highly alkaline solutions and related applications. IBPs, like most proteins, are sensitive to changes in their physical environment, which can alter their physical and chemical properties and ice-binding activities. Here we describe how to investigate IBP integrity for applications in alkaline environments and discuss incorporating IBPs into portland cement paste, an alkaline ceramic, to assess the reduction of ice content and mitigation of freeze-thaw damage to the brittle cementitious matrix.

Keywords: Alkaline materials; Cement paste; Freeze-thaw resistance; IBP applications; IBP stability.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Caspase 1
Ceramics*
Environment
Ice*

Substances

Ice
Caspase 1