Measurement of Ice Nucleation Activity of Biological Samples

Rosemary J Eufemio; Ralph Schwidetzky; Konrad Meister

doi:10.1007/978-1-0716-3503-2_7

Measurement of Ice Nucleation Activity of Biological Samples

Methods Mol Biol. 2024:2730:101-107. doi: 10.1007/978-1-0716-3503-2_7.

Authors

Rosemary J Eufemio¹, Ralph Schwidetzky², Konrad Meister^{3

4

5}

Affiliations

¹ Biomolecular Sciences Graduate Program, Boise State University, Boise, ID, USA.
² Max Planck Institute for Polymer Research, Mainz, Germany.
³ Biomolecular Sciences Graduate Program, Boise State University, Boise, ID, USA. konradmeister@boisestate.edu.
⁴ Max Planck Institute for Polymer Research, Mainz, Germany. konradmeister@boisestate.edu.
⁵ Department of Chemistry and Biochemistry, Boise State University, Boise, ID, USA. konradmeister@boisestate.edu.

PMID: 37943453
DOI: 10.1007/978-1-0716-3503-2_7

Abstract

Experimentation with ice-nucleating biomolecules is needed to advance the fundamental understanding of biotic heterogeneous ice nucleation. Standard experimental procedures vary with sample type. Here we describe a generalized primary purification and analysis process to measure ice nucleation activity of biological samples using an advanced freezing droplet assay.

Keywords: Heterogeneous ice nucleation; Ice-nucleating biomolecules.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Biological Assay*
Ice*
Research Design

Substances

Ice