Generation of induced pluripotent stem cell lines from a sister pair who suffered post-partum or recurrent Spontaneous Coronary Artery Dissections

Monique Bax; Keerat Junday; Siiri E Iismaa; Xenia Kaidonis; David Muller; Stephanie Hesselson; Robert M Graham

doi:10.1016/j.scr.2023.103238

Generation of induced pluripotent stem cell lines from a sister pair who suffered post-partum or recurrent Spontaneous Coronary Artery Dissections

Stem Cell Res. 2023 Dec:73:103238. doi: 10.1016/j.scr.2023.103238. Epub 2023 Oct 30.

Authors

Monique Bax¹, Keerat Junday², Siiri E Iismaa¹, Xenia Kaidonis¹, David Muller³, Stephanie Hesselson², Robert M Graham⁴

Affiliations

¹ Victor Chang Cardiac Research Institute, Darlinghurst 2010, Australia; University of New South Wales Sydney, Kensington, New South Wales 2052, Australia.
² Victor Chang Cardiac Research Institute, Darlinghurst 2010, Australia.
³ Victor Chang Cardiac Research Institute, Darlinghurst 2010, Australia; St Vincent's Hospital, Darlinghurst, New South Wales 2010, Australia; University of New South Wales Sydney, Kensington, New South Wales 2052, Australia.
⁴ Victor Chang Cardiac Research Institute, Darlinghurst 2010, Australia; St Vincent's Hospital, Darlinghurst, New South Wales 2010, Australia; University of New South Wales Sydney, Kensington, New South Wales 2052, Australia. Electronic address: B.Graham@victorchang.edu.au.

PMID: 37939621
DOI: 10.1016/j.scr.2023.103238

Abstract

Spontaneous Coronary Artery Dissection (SCAD) results from a bleed within a coronary artery wall that impairs blood flow as it expands. It is the most common cause of myocardial infarction in pregnant women. Here, peripheral blood mononuclear cells from two sisters who had suffered SCADs were reprogrammed using Sendai Virus. Expression of pluripotency markers, capability to differentiate to the three germ layers, and cellular integrity were confirmed. This is the first report of a SCAD family induced pluripotent stem cell (iPSC) cohort, including a sister who suffered post-partum SCAD, and one who suffered from multiple recurrences.

MeSH terms

Coronary Vessels
Female
Humans
Induced Pluripotent Stem Cells* / metabolism
Leukocytes, Mononuclear
Postpartum Period
Pregnancy

Supplementary concepts

Coronary Artery Dissection, Spontaneous