Comparative Study of Liposomal and Ethosomal Formulations of Curcuma heyneana Rhizome Extract in a Transdermal Delivery System

Idha Kusumawati; Kresma Oky Kurniawan; Rohmania Rohmania; Bernasdito Ade Pratama; Yusuf Alif Pratama; Subhan Rullyansyah; Mega Ferdina Warsito; Retno Widyowati; Eka Pramyrtha Hestianah; Katsuyoshi Matsunami

doi:10.2174/0122117385252518231018161755

Comparative Study of Liposomal and Ethosomal Formulations of Curcuma heyneana Rhizome Extract in a Transdermal Delivery System

Pharm Nanotechnol. 2023 Nov 2. doi: 10.2174/0122117385252518231018161755. Online ahead of print.

Affiliations

¹ Department of Pharmaceutical Science, Faculty of Pharmacy, Universitas Airlangga, Nanizar Zaman Joenoes Building, Surabaya 60155, Indonesia.
² Natural Product Drug Discovery and Development Research Group, Faculty of Pharmacy, Universitas Airlangga, Nanizar Zaman Joenoes Building, Surabaya 60155, Indonesia.
³ Research Centre for Applied Microbiology, National Research and Innovation Agency (BRIN), Jl. Raya Jakarta-Bogor Km 46, Cibinong 16911, Bogor, Indonesia.
⁴ Veterinary Anatomy Department, Faculty of Veterinary, Universitas Airlangga, Jl. Mulyorejo, Surabaya 60155, Indonesia.
⁵ Department of Pharmacognosy, Graduate School of Biomedical & Health Sciences, Hiroshima University, 1-2-3, Kasumi, Minami-ku, Hiroshima, Japan.

PMID: 37937575
DOI: 10.2174/0122117385252518231018161755

Abstract

Aim: This study aimed to develop an anti-aging nanoformulation with Curcuma heyneana extract as bioactive substance.

Background: Curcuma heyneana Valeton & Zipj extract has been proven in previous research to have antioxidant, anti-ageing, anti-inflammatory, and wound healing properties, which makes it a potential bioactive material for anti-ageing and sunscreen cosmetic products. Phytoantioxidants need to penetrate into deeper skin layers to ensure effectivity. Thus, a transdermal delivery system is needed to deliver the extract to a deeper skin layer.

Objective: The objective of the study was to compare the permeability and anti-ageing activity of liposomal and ethosomal formulations of C. heynena rhizome ethanolic extract.

Methods: In this study, C. heyneana extract was loaded into a phospholipid vesicular system in the form of liposome and ethosome formulations using the ethanolic injection method. The anti-ageing activity was assessed by analyzing the epidermal thickness, number of sunburn cells, distance between collagen fibres, and number of fibroblasts. While the histologic specimen scoring was carried out for the in vivo penetration study.

Results: The ethosomal formulation had been found to have better penetration ability since it was able to reach the lower dermis area compared to the liposomes, which only reached the upper dermis. The ethosomal formulation of C. heyneana extract exhibited a better anti-ageing activity based on the parameters of epidermal thickness, sunburn cell count, fibroblast count, and the distance between collagen fibres in rat skin histology.

Conclusion: Ethosomes have been found to be a more proficient carrier system for transdermal delivery of C. heyneana extract compared to liposomes. Meanwhile, their penetration correlated with the effectivity of the formulation, suggesting that the vesicular system enhanced the penetration ability of the extract.

Keywords: Curcuma heyneana; ethosome; liposome; medicinCurcuma heyneana; medicine; penetration; phospholipid; transdermal delivery.