Bacterial profile and extended spectrum beta lactamase screening of urinary tract infection among asymptomatic and symptomatic pregnant women attending antenatal care in ALERT Hospital, Addis Ababa, Ethiopia

Molla Getie; Solomon Gebre-Selassie; Yemeserach Getu; Setognal Birara; Chalachew Tiruneh; Abtie Abebaw; Yibeltal Akelew; Getu Abeje; Aklesya Enkobahry

doi:10.1177/20503121231197587

Bacterial profile and extended spectrum beta lactamase screening of urinary tract infection among asymptomatic and symptomatic pregnant women attending antenatal care in ALERT Hospital, Addis Ababa, Ethiopia

SAGE Open Med. 2023 Nov 3:11:20503121231197587. doi: 10.1177/20503121231197587. eCollection 2023.

Authors

Molla Getie¹, Solomon Gebre-Selassie², Yemeserach Getu³, Setognal Birara⁴, Chalachew Tiruneh⁵, Abtie Abebaw⁶, Yibeltal Akelew⁶, Getu Abeje⁷, Aklesya Enkobahry⁵

Affiliations

¹ Departments of Medical Laboratory Science, Injibara University, Injibara, Ethiopia.
² Department of Microbiology, Immunology and Parasitology, Addis Ababa University, Addis Ababa, Ethiopia.
³ Department of Medical Laboratory/Microbiology Unit, ALERT Hospital, Addis Ababa, Ethiopia.
⁴ Department of Epidemiology and Biostatics', University of Gonder, Gonder, Ethiopia.
⁵ Departments of Biomedical Science, Injibara University, Injibara, Ethiopia.
⁶ Department of Medical Laboratory Science, Debere Markose University, Debere Markose, Ethiopia.
⁷ Departments of Biomedical Science, Samara University, Samara, Ethiopia.

Abstract

Introduction: The occurrence of extended spectrum beta lactamase-producing uropathogens, especially in pregnant women can result in life-threatening condition and morbidity for both the mother and the newborn due to very limited drug options for treatment of these pathogens. The aim of this study was to determine the bacterial profile, associated factors, and their antimicrobial susceptibility patterns and to identify extended spectrum beta lactamase-producing bacterial uropathogens.

Methods: A hospital-based cross-sectional study was conducted from July to September 2018 on a total of 177 pregnant women with and without symptoms of urinary tract infection at ALERT Hospital, Addis Ababa, Ethiopia. From these study participants, 72 have symptoms, whereas 105 have no symptoms. All urine samples were inoculated onto cysteine lactose electrolyte deficient medium and MacConkey agar. Colonies were counted to check the presence of significant bacteriuria. Pure isolates of bacterial pathogen were characterized and identified at species level by colony morphology, gram stain, and standard biochemical procedures. All Gram-negative isolates were put into Muller-Hinton agar plates for antibiotic susceptibility test by Kirby-Bauer disc diffusion technique. Extended spectrum beta lactamase was detected using double-disk synergy methods on Muller-Hinton agar. The data were double entered into epidemiological Information system and analyzed using Statistical Package for Social Science version 26.

Results: The overall proportion of urinary tract infection among pregnant women was 14.7% (n = 26/177). Klebsiella pneumoniae was the predominant bacterial etiologic agent of urinary tract infection 26.9% (n = 7/26). The proportion of extended spectrum beta lactamase among Gram-negative isolates was 50% (n = 6/12). Among extended spectrum beta lactamase-producing isolates (100%), all are resistance to amikacin and gentamicin while intermediate level resistance rate of 66.7% was observed among trimethoprim-sulphamethoxazole. They were susceptible for some limited drugs, and these were Nitrofurantoin (83.3%) and Chloramphenicol (83.3%).

Conclusions: Majority of extended spectrum beta lactamase-producing isolates exhibited co-resistance to other commonly prescribed antibiotics. This indicates that the option of treatment for these pathogens rapidly decreased from time to time which results serious life-threatening conditions, especially in mother and newborn unless the appropriate measure is taken.

Keywords: Addis Ababa; Ethiopia; Extended spectrum beta lactamases; antibiotic resistance; pregnancy; urinary tract infection; uropathogen.