Genome-driven discovery of new serrawettin W2 analogues from Serratia fonticola DSM 4576

Haolin Qiu; Yang Xiao; Ling Shen; Tao Han; Qiang He; Aiying Li; Peng Zhang; Xiaofeng Cai

doi:10.1039/d3ob01642k

Genome-driven discovery of new serrawettin W2 analogues from Serratia fonticola DSM 4576

Org Biomol Chem. 2023 Nov 22;21(45):9029-9036. doi: 10.1039/d3ob01642k.

Authors

Haolin Qiu¹, Yang Xiao¹, Ling Shen¹, Tao Han¹, Qiang He², Aiying Li³, Peng Zhang¹, Xiaofeng Cai^{1

4}

Affiliations

¹ School of Pharmacy, Tongji Medical College of Huazhong University of Science and Technology, Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, Wuhan 430030, People's Republic of China. caixiaofeng@hust.edu.cn.
² Xianning Public Inspection Center of Hubei Province, Xianning 437000, P. R. China.
³ Helmholtz International Lab for Anti-Infectives, Shandong University-Helmholtz Institute of Biotechnology, State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, P.R. China.
⁴ State Key Laboratory of Dao-di Herbs, Beijing 100700, P. R. China.

PMID: 37930431
DOI: 10.1039/d3ob01642k

Abstract

By expressing a multimodular NRPS gene sefA from Serratia fonticola DSM 4576 in E. coli, four new serrawettin W2 analogues, namely sefopeptides A-D (1-4), were isolated and structurally characterized and their biosynthesis was proposed. A bioactivity assay showed that sefopeptide C (3) exhibits moderate cytotoxic activity against acute promyelocytic leukemia NB4 cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Escherichia coli* / genetics
Humans
Leukemia, Promyelocytic, Acute*
Peptides, Cyclic / chemistry
Serratia / genetics

Substances

serrawettin W2
Peptides, Cyclic

Supplementary concepts

Serratia fonticola