Screening and evaluation of skin potential probiotic from high-altitude Tibetans to repair ultraviolet radiation damage

Zhihao Zhang; Haixia Ran; Yutong Hua; Feilong Deng; Bo Zeng; Jianmin Chai; Ying Li

doi:10.3389/fmicb.2023.1273902

Screening and evaluation of skin potential probiotic from high-altitude Tibetans to repair ultraviolet radiation damage

Front Microbiol. 2023 Oct 19:14:1273902. doi: 10.3389/fmicb.2023.1273902. eCollection 2023.

Authors

Zhihao Zhang^{1

2}, Haixia Ran³, Yutong Hua⁴, Feilong Deng^{1

2}, Bo Zeng⁴, Jianmin Chai^{1

2}, Ying Li^{1

2}

Affiliations

¹ Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, College of Life Science and Engineering, Foshan University, Foshan, China.
² School of Life Science and Engineering, Foshan University, Foshan, China.
³ Animal Husbandry and Fisheries Technology Extension Station, Chongqing, China.
⁴ Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, China.

Abstract

Human skin microbes play critical roles in skin health and diseases. Microbes colonizing on the skin of Tibetans living in the high-altitude area for generations may have a stronger ability to resist the harsh environment, such as high ultraviolet radiation (UV). Isolation of a potential probiotic from Tibetans skin is beneficial for resistance of skin disease for humans in the world. In this study, the signature microbiota for Tibetan skin were characterized compared to low-altitude humans. Next, using culture-omics, 118 species were isolated. The culturability of high-altitude of Tibetan skin microbiome reached approximate 66.8%. Next, we found that one strain, Pantoea eucrina, had the greatest ability to repair UV damage to the skin as the lowest pathological score was observed in this group. Interestingly, another animal trial found this bacterium resisted UV rather than its metabolites. Using whole genome sequencing, this strain P. eucrina KBFS172 was confirmed, and its functions were annotated. It might involve in the metabolic pathway of carotenoid biosynthesis with anti-oxidative stress properties, which plays critical roles in UV-damage repair. In conclusion, we characterized the signature microbes of skin in high-altitude Tibetans, isolated a skin bacterium of Pantoea eucrina KBFS172 which could repair UV damage via involving the metabolic pathway of carotenoid biosynthesis. Our results provide a new potential skin probiotic for skin disease prevention or sunburn.

Keywords: UV damage; culturomics; high altitude; probiotic; skin disease; skin microbiota.

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This project was supported by National Natural Science Foundation of China (32170430), Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding (2019B030301010), and Key Laboratory of Animal Molecular Design and Precise Breeding of Guangdong Higher Education Institutes (2019KSYS011).