Glycosyl hydrolase from Pseudomonas fluorescens inhibits the biofilm formation of Pseudomonads

Di Wang; Syed Tatheer Alam Naqvi; Fanglin Lei; Zhenyu Zhang; Haiying Yu; Luyan Z Ma

doi:10.1016/j.bioflm.2023.100155

Glycosyl hydrolase from Pseudomonas fluorescens inhibits the biofilm formation of Pseudomonads

Biofilm. 2023 Sep 16:6:100155. doi: 10.1016/j.bioflm.2023.100155. eCollection 2023 Dec 15.

Authors

Di Wang¹, Syed Tatheer Alam Naqvi¹, Fanglin Lei^{1

2}, Zhenyu Zhang^{1

3}, Haiying Yu¹, Luyan Z Ma^{1

3}

Affiliations

¹ State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, PR China.
² Yunnan University, Kunming, 650500, PR China.
³ University of Chinese Academy of Sciences, Beijing, 100049, PR China.

Abstract

Biofilms are complex microbial communities embedded in extracellular matrix. Pathogens within the biofilm become more resistant to the antibiotics than planktonic counterparts. Novel strategies are required to encounter biofilms. Exopolysaccharides are one of the major components of biofilm matrix and play a vital role in biofilm architecture. In previous studies, a glycosyl hydrolase, PslG_PA, from Pseudomonas aeruginosa was found to be able to inhibit biofilm formation by disintegrating exopolysaccharide in biofilms. Here, we investigate the potential spectrum of PslG homologous protein with anti-biofilm activity. One glycosyl hydrolase from Pseudomonas fluorescens, PslG_PF, exhibits anti-biofilm activities and the key catalytic residues of PslG_PF are conserved with those of PslG_PA. PslG_PF at concentrations as low as 50 nM efficiently inhibits the biofilm formation of P. aeruginosa and disassemble its preformed biofilm. Furthermore, PslG_PF exhibits anti-biofilm activity on a series of Pseudomonads, including P. fluorescens, Pseudomonas stutzeri and Pseudomonas syringae pv. phaseolicola. PslG_PF stays active under various temperatures. Our findings suggest that P. fluorescens glycosyl hydrolase PslG_PF has potential to be a broad spectrum inhibitor on biofilm formation of a wide range of Pseudomonads.

Keywords: Anti-biofilm; Exopolysaccharides; Glycosyl hydrolase; Pseudomonas.