Majority of dyes are toxic to all the living organisms and inherently resistant to microbial degradation. Hence, decolorization and degradation of textile dye methyl red were evaluated using isolated bacterial strain Pseudomonas aeruginosa (P. aeruginosa). Methyl red dye decolorization by P. aeruginosa with respect to various parameters was optimized. Data shows that maximum possible decolorization was seen at 50 ppm dye concentration, 1400 mg/l glucose concentration, 700 mg/l sodium chloride (NaCl) concentration, pH 9, temperature 38°C, 1000 mg/l urea concentration P. aeruginosa AM-1 strain. The highest percent (91.1%) of bioremediation was achieved at 40 ppm dye concentration in Allium cepa test. These findings suggest P. aeruginosa strain (AM-1) has the potential to be used in the biological treatment of highly toxic dye which is main constituent of dyeing mill effluents due to its high decolorization activity with simple conditions. Strain AW-1 strain also has potential to bioremediate other wastewater containing methyl red dye.
Keywords: Bioremediation; Decolorization; Methyl red dyes; Pseudomonas aeruginosa; Wastewater.
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