Determining the safety and efficacy of dietary supplementation with 3'-sialyllactose or 6'-sialyllactose on growth, tolerance, and brain sialic acid concentrations

Rebecca K Golden; Loretta T Sutkus; Laura L Bauer; Sharon M Donovan; Ryan N Dilger

doi:10.3389/fnut.2023.1278804

Determining the safety and efficacy of dietary supplementation with 3'-sialyllactose or 6'-sialyllactose on growth, tolerance, and brain sialic acid concentrations

Front Nutr. 2023 Oct 19:10:1278804. doi: 10.3389/fnut.2023.1278804. eCollection 2023.

Authors

Rebecca K Golden¹, Loretta T Sutkus¹, Laura L Bauer², Sharon M Donovan^{3

4}, Ryan N Dilger^{1

2

4}

Affiliations

¹ Neuroscience Program, University of Illinois, Urbana, IL, United States.
² Department of Animal Sciences, University of Illinois, Urbana, IL, United States.
³ Department of Food Science and Human Nutrition, University of Illinois, Urbana, IL, United States.
⁴ Division of Nutritional Sciences, University of Illinois, Urbana, IL, United States.

Abstract

Sialylated oligosaccharides, including 3'-sialyllactose (3'-SL) and 6'-sialyllactose (6'-SL), comprise a large portion of human milk and have been known to support development over the first year of life. While research has investigated the impact of early-life supplementation, longer-term supplementation remains relatively unexplored. Consequently, the following study assesses the impact of supplementation of either 3'-SL or 6'-SL on growth performance, tolerance, and brain sialic acid concentrations. Two-day-old piglets (n = 75) were randomly assigned to a commercial milk replacer ad libitum without or with 3'-SL or 6'-SL (added at 0.2673% on an as-is basis). Daily body weight and feed disappearance were recorded to assess growth performance and tolerance. Pigs were euthanized for sample collection on postnatal day 33 (n = 30) or 61 (n = 33), respectively. Across growth performance, clinical chemistry and hematology, histomorphology, and sialic acid quantification, dietary differences were largely unremarkable at either time-point. Overall, SA was well-tolerated both short-term and long-term.

Keywords: 3ˊ-sialyllactose; 6ˊ-sialyllactose; growth; hematology; histomorphology; sialic acid.

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. Kyowa Hakko Bio CO., LTD., Tokyo, Japan, funded this research. After initial contributions in study design, the funders did not participate in sample collection, data analysis, interpretation of data, or writing of the manuscript.