Sathuperi virus (SATV) and Shamonda virus (SHAV) (family Peribunyaviridae, genus Orthobunyavirus, species Schmallenberg orthobunyavirus) have been suggested to cause congenital abnormalities in ruminants. In this study, we determined the complete genome sequences of SATV KSB-6/C/02 and SHAV KSB-2/C/08 strains, which were obtained from Culicoides biting midges in Japan, by next-generation sequencing and Sanger sequencing. The 3'- and 5'-untranslated region sequences of the M segment of SHAV KSB-2/C/08 strains are distinctly different from those of SATV KSB-6/C/02 and Schmallenberg viruses. This study provides the genome characterization of Japanese strains of SATV and SHAV and presented the genetic variation in the untranslated regions of Schmallenberg orthobunyavirus M segments.
Keywords: Sathuperi virus; Shamonda virus; complete genome sequence.