Berberine is a natural isoquinoline alkaloid performing wide-spectrum antimicrobial and antiviral effects like antibiotics. Its production generates berberine containing wastewater, and berberine adsorbed on waste activated sludge (WAS) will unavoidably enter the anaerobic digestion (AD) system while its impact on the AD process is unknown. Our research found that berberine of 20 mg/L (BBR20) slightly enhanced the methane yield (4.2 ± 0.6%) under mesophilic condition (35.0 ± 1.0 °C). However, 100 and 500 mg/L (BBR100 and BBR500) depressed methane production by 17.3 ± 4.3% and 83.2 ± 0.4%; meanwhile more soluble chemical oxygen demand (SCOD) including volatile fatty acid (VFA), protein, and polysaccharide were left in the fermentation broth, which led to an increase in sludge reduction. 88.3 ± 0.09%-99.1 ± 0.04% of berberine was distributed in the sludge phase and could be efficiently removed even under a high berberine level of 500 mg/L during the AD process. Exposure to different berberine concentrations promoted sludge dissolution and triggered more sludge extracellular polymeric substances (EPS) being dissolved. Lower berberine concentration (20 mg/L) enhanced acidification and methanogenesis steps, resulting in a final methane generation increase. While hydrolysis, acidification and methanogenesis processes were all inhibited by 100 and 500 mg/L berberine. Microbial analysis revealed that the main acid-producing bacteria genera were changed as Bacteroidetes vadinHA17 dominated in control, BBR20 and BBR100 groups, was replaced by Petrimonas in BBR500. Additionally, Methanosaeta, as a strict acetoclastic methanogen, was suppressed under exposure to 100 and 500 mg/L berberine. Accordingly, the declined abundance of archaea genera consuming acetic acid caused more VFA accumulation and less methane production in BBR100 and BBR500 groups.
Keywords: Anaerobic digestion; Berberine; Methane production; Microbial community; Waste activated sludge.
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