Multicolor detection of glutathione by manganese dioxide nanosheets and gold nanotetrapods based on an anti-etching mechanism

Shengwen Wang; Wen Zang; Minjie Peng; Lijing Miao; Aiguo Wu; Yujie Zhang

doi:10.1016/j.talanta.2023.125366

Multicolor detection of glutathione by manganese dioxide nanosheets and gold nanotetrapods based on an anti-etching mechanism

Talanta. 2024 Feb 1;268(Pt 2):125366. doi: 10.1016/j.talanta.2023.125366. Epub 2023 Oct 29.

Authors

Shengwen Wang¹, Wen Zang², Minjie Peng², Lijing Miao², Aiguo Wu³, Yujie Zhang⁴

Affiliations

¹ Ningbo Key Laboratory of Biomedical Imaging Probe Materials and Technology, Zhejiang International Cooperation Base of Biomedical Materials Technology and Application, Chinese Academy of Sciences (CAS) Key Laboratory of Magnetic Materials and Devices, Ningbo Cixi Institute of Biomedical Engineering, Zhejiang Engineering Research Center for Biomedical Materials, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo, 315201, China; School of Materials Science and Chemical Engineering, Ningbo University, Ningbo, 315211, China.
² Ningbo Key Laboratory of Biomedical Imaging Probe Materials and Technology, Zhejiang International Cooperation Base of Biomedical Materials Technology and Application, Chinese Academy of Sciences (CAS) Key Laboratory of Magnetic Materials and Devices, Ningbo Cixi Institute of Biomedical Engineering, Zhejiang Engineering Research Center for Biomedical Materials, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo, 315201, China.
³ Ningbo Key Laboratory of Biomedical Imaging Probe Materials and Technology, Zhejiang International Cooperation Base of Biomedical Materials Technology and Application, Chinese Academy of Sciences (CAS) Key Laboratory of Magnetic Materials and Devices, Ningbo Cixi Institute of Biomedical Engineering, Zhejiang Engineering Research Center for Biomedical Materials, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo, 315201, China; University of Chinese Academy of Sciences, Beijing, 100049, China. Electronic address: aiguo@nimte.ac.cn.
⁴ Ningbo Key Laboratory of Biomedical Imaging Probe Materials and Technology, Zhejiang International Cooperation Base of Biomedical Materials Technology and Application, Chinese Academy of Sciences (CAS) Key Laboratory of Magnetic Materials and Devices, Ningbo Cixi Institute of Biomedical Engineering, Zhejiang Engineering Research Center for Biomedical Materials, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo, 315201, China; University of Chinese Academy of Sciences, Beijing, 100049, China. Electronic address: zhangyujie@nimte.ac.cn.

PMID: 37925881
DOI: 10.1016/j.talanta.2023.125366

Abstract

Glutathione (GSH) is a crucial non-protein thiol and an indispensable endogenous antioxidant. The aberrant expression of GSH in plasma and cytosol is closely related to numerous diseases, including cancer. Therefore, establishing a sensitive method for analyzing GSH has important application value for biomedical research and clinical medical detection. Herein, A method for the rapid and simple detection of GSH was proposed, which is based on an anti-etching mechanism by utilizing gold nanotetrapods (Au NTPs) and manganese dioxide nanosheets (MnO₂ NSs). In the absence of GSH, Au NTPs solution can cause a distinct color change from gray-green to red through the etching effect of MnO₂ NSs. However, in the presence of GSH, the redox reaction between GSH and MnO₂ NSs inhibits the etching of Au NTPs by MnO₂ NSs, and Au NTPs solution maintains persistent gray-green color. The colorimetric probe exhibited excellent selectivity for GSH. The limits of detection for GSH were 43.5 nM (UV-vis spectrum) and 0.25 μM (naked eyes). The sensing technique exhibited excellent linearity between wavelength shift and GSH concentration within the range of 0.25 μM-1.5 μM. The outcomes of GSH detection in actual biological samples demonstrate that this probe has the potential to be applied to GSH detection in intricate biological samples.

Keywords: Anti-etching mechanism; GSH colorimetric detection; Gold nanotetrapods; Manganese dioxide nanosheets; Multicolor response.

MeSH terms

Glutathione
Gold
Limit of Detection
Manganese Compounds
Metal Nanoparticles*
Oxides*

Substances

manganese dioxide
Oxides
Manganese Compounds
Gold
Glutathione