Pinocembrin attenuates susceptibility to atrial fibrillation in rats with pulmonary arterial hypertension

Eur J Pharmacol. 2023 Dec 5:960:176169. doi: 10.1016/j.ejphar.2023.176169. Epub 2023 Nov 2.

Abstract

Background: Pulmonary arterial hypertension (PAH) is a disease characterized by pulmonary vascular remodeling that triggers fibrosis and excessive myocardium apoptosis, ultimately facilitating atrial fibrillation (AF). In various rat models, Pinocembrin has anti-fibrotic and anti-apoptotic effects, reducing arrhythmia vulnerability. However, whether pinocembrin alleviates to AF in a PAH model remains unclear. The experiment aims to investigate how pinocembrin affects AF susceptibility in PAH rats and the possible mechanisms involved.

Methods: The PAH model was induced by monocrotaline (MCT; i. p. 60 mg/kg). Concurrently, rats received pinocembrin (i.p.50 mg/kg) or saline. Hemodynamics parameters, electrocardiogram parameters, lung H.E. staining, atrial electrophysiological parameters, histology, Western blot, and TUNEL assay were detected.

Results: Compared to the control rats, MCT-induced PAH rats possessed prominently enhancive mPAP (mean pulmonary artery pressure), pulmonary vascular remodeling, AF inducibility, HRV, right atrial myocardial fibrosis, apoptosis, atrial ERP, APD, and P-wave duration. Additionally, there were lowered protein levels of Cav1.2, Kv4.2, Kv4.3, and connexin 40 (CX40) in the MCT group in right atrial tissue. However, pinocembrin reversed the above pathologies and alleviated the activity of the Rho A/ROCKs signaling pathway, including the expression of Rho A, ROCK1, ROCK2, and its downstream MYPT-1, LIMK2, BCL-2, BAX, cleaved-caspase3 in right atrial and HL-1 cells.

Conclusion: Present data exhibited pinocembrin attenuated atrial electrical, ion-channel, and autonomic remodeling, diminished myocardial fibrosis and apoptosis levels, thereby reducing susceptibility to AF in the MCT-induced PAH rats. Furthermore, we found that pinocembrin exerted inhibitory action on the Rho A/ROCK signaling pathway, which may be potentially associated with its anti-AF effects.

Keywords: Apoptosis; Atrial fibrillation; Electrophysiology; Monocrotaline; Pinocembrin; Pulmonary arterial hypertension.

MeSH terms

  • Animals
  • Atrial Fibrillation* / chemically induced
  • Atrial Fibrillation* / drug therapy
  • Atrial Fibrillation* / pathology
  • Disease Models, Animal
  • Familial Primary Pulmonary Hypertension / pathology
  • Fibrosis
  • Monocrotaline / pharmacology
  • Pulmonary Arterial Hypertension* / chemically induced
  • Pulmonary Arterial Hypertension* / drug therapy
  • Pulmonary Arterial Hypertension* / pathology
  • Pulmonary Artery / pathology
  • Rats
  • Rats, Sprague-Dawley
  • Vascular Remodeling

Substances

  • pinocembrin
  • Monocrotaline