In the present study, the efficacy of one-step direct transesterification (OSDT) and Dual-step extraction-transesterification (DSET) of Chlorococcum sp., Nannochloropsis sp., and their consortium was evaluated for fatty acid methyl ester (FAME) yield. Initially, the biomass yield and lipid content of the two strains and their consortium were estimated. Of the biomasses, the consortium showed a higher biomass yield of 1.41 g/L and lipid content of 30.2%, which is higher than the monocultures irrespective of the different biomass drying methods used. With regards to the FAME yield, OSDT and DSET have yielded almost similar quantities about 21 g/100g dried biomass. Of the different reaction conditions of OSDT tested, a higher FAME yield at 70-71% (based on lipid weight) was obtained at 75 °C reaction temperature, 3 h reaction time with a 2g sample size. Eventually, the fatty acid composition of consortium biomass revealed higher levels of saturated and monounsaturated fatty acids in the vicinity of 46 and 25%, respectively. Based on the results, it is concluded that OSDT is a promising method due to its low energy consumption, cost-effective and time-saving attributes for quality biodiesel production from the Chlorococcum-Nannochloropsis consortium.
Keywords: Biodiesel; Direct esterification; Fatty acids; In situ transesterification; Microalgae; Nannochloropsis.
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