Implications of albumin in cell culture media on the biological action of vanadates(V)

Abstract

In this article, the implications of binding competition of vanadates(V) with dodecyl sulfates for bovine serum albumin on cytotoxicity of vanadium(V) species against prostate cancer cells have been investigated. The pH- and SDS-dependent vanadate(V)-BSA interactions were observed. At pH 5, there is only one site capable of binding ten vanadates(V) ions (logK_(ITC)1 = 4.96 ± 0.06; ΔH_(ITC)1 = -1.04 ± 0.03 kcal mol^-1), whereas at pH 7 two distinctive binding sites on protein were found, saturated with two and seven V(V) ions, respectively (logK_(ITC)1 = 6.11 ± 0.06; ΔH_(ITC)1 = 0.78 ± 0.12 kcal mol^-1; logK_(ITC)2 = 4.80 ± 0.02; ΔH_(ITC)2 = - 4.95 ± 0.14 kcal mol^-1). SDS influences the stoichiometry and the stability of the resulting V(V)-BSA complexes. Finally, the cytotoxicity of vanadates(V) against prostate cancer cells (PC3 line) was examined in the presence and absence of SDS in the culture medium. In the case of a 24-h incubation with 100 μM vanadate(V), a ca. 20 % reduction in viability of PC3 cells was observed in the presence of SDS. However, in other considered cases (various concentrations and time of incubation) SDS does not affect the dose-dependent action of vanadates(V) on the investigated prostate cancer cells.

Keywords: Bovine serum albumin; Prostate cancer cells; Vanadate(V).