Water stress significantly affects the diurnal variation of solar-induced chlorophyll fluorescence (SIF): A case study for winter wheat

Abstract

Remote sensing of Solar-induced chlorophyll fluorescence (SIF) has been widely used in estimating Gross Primary Productivity (GPP) and detecting stress in terrestrial ecosystems. Water stress adversely impacts the growth, development, and productivity of a plant. Recently, the characterizing and understanding of the diurnal cycling of plant functioning and ecosystem processes has been explored using SIF. However, the diurnal response of SIF to different levels of water stress remains unclear. This study conducted field experiments on winter wheat by subjecting it to different levels of water stress including well-watered (CK) and, mild, moderate, and severe water stress (D1, D2, D3), and collected the spectral data using an automated SIF measurement system. The results observed the strong SIF-PAR (photosynthetically active radiation) correlations and that these relationships gradually decoupled with increasing water stress, which further decreased the accuracy of temporal upscaling of far-red SIF from an instantaneous to daily scale. To quantify the characteristics of diurnal far-red SIF, five indices including peak time, peak value, curve opening coefficient (leading coefficient of the parabola), and left/right slopes of the peak were proposed. The results demonstrated that diurnal far-red SIF was characterized by an earlier peak time, decreasing peak value, wider curve opening, and flattening right slope from the CK plot to the D3 plot. There were certain mechanisms linking the different indices, for example, between peak size and opening coefficient. Furthermore, the response of far-red SIF to water stress was most pronounced at noon. SIF/PAR exhibited a more significant response to varying water stress compared to far-red SIF, which mitigated the negative influence of PAR variations on diurnal SIF. These findings contribute to the monitoring of plant water dynamics at fine temporal scales.

Keywords: Diurnal variation; Solar-induced chlorophyll fluorescence; Water stress; Winter wheat.