Microbially driven Fe-N cycle: Intrinsic mechanisms, enhancement, and perspectives

Abstract

The iron‑nitrogen (FeN) cycle driven by microbes has great potential for treating wastewater. Fe is a metal that is frequently present in the environment and one of the crucial trace elements needed by microbes. Due to its synergistic role in the microbial N removal process, Fe goes much beyond the essential nutritional needs of microorganisms. Investigating the mechanisms behind the linked Fe-N cycle driven by microbes is crucial. The Fe-N cycle is frequently connected with anaerobic ammonia oxidation (anammox), nitrification, denitrification, dissimilatory nitrate reduction to ammonium (DNRA), Feammox, and simultaneous nitrification denitrification (SND), etc. Although the main mechanisms of Fe-mediated biological N removal may vary depending on the valence state of the Fe, their similar transformation pathways may provide information on the study of certain element-microbial interactions. This review offers a thorough analysis of the facilitation effect and influence of Fe on the removal of nitrogenous pollutants in various biological N removal processes and summarizes the ideal Fe dosing. Additionally, the synergistic mechanisms of Fe and microbial synergistic N removal process are elaborated, covering four aspects: enzyme activity, electron transfer, microbial extracellular polymeric substances (EPS) secretion, and microbial community interactions. The methods to improve biological N removal based on the intrinsic mechanism were also discussed, with the aim of thoroughly understanding the biological mechanisms of Fe in the microbial N removal process and providing a reference and thinking for employing Fe to promote microbial N removal in practical applications.

Keywords: Biological nitrogen removal; Fe-N coupling cycle; Feammox; Intrinsic mechanisms; Process enhancement.