Synergy of iron-natural phenolic microparticles and hydrophobic ionic liquid for enrichment of tetracycline residues in honey prior to HPLC-UV detection

Tammanoon Nilnit; Janjira Jeenno; Sam-Ang Supharoek; Jitlada Vichapong; Watsaka Siriangkhawut; Kraingkrai Ponhong

doi:10.1016/j.foodchem.2023.137879

Synergy of iron-natural phenolic microparticles and hydrophobic ionic liquid for enrichment of tetracycline residues in honey prior to HPLC-UV detection

Food Chem. 2024 Mar 30;437(Pt 1):137879. doi: 10.1016/j.foodchem.2023.137879. Epub 2023 Oct 26.

Authors

Tammanoon Nilnit¹, Janjira Jeenno¹, Sam-Ang Supharoek², Jitlada Vichapong³, Watsaka Siriangkhawut¹, Kraingkrai Ponhong⁴

Affiliations

¹ Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Mahasarakham University, Maha Sarakham 44150, Thailand.
² Department of Medical Science, Amnatcharoen Campus, Mahidol University, Amnat Charoen 37000, Thailand; Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand. Electronic address: samang.sup@mahidol.ac.th.
³ Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Mahasarakham University, Maha Sarakham 44150, Thailand; Multidisciplinary Research Unit of Pure and Applied Chemistry, Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Mahasarakham University, Maha Sarakham 44150, Thailand.
⁴ Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Mahasarakham University, Maha Sarakham 44150, Thailand; Multidisciplinary Research Unit of Pure and Applied Chemistry, Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Mahasarakham University, Maha Sarakham 44150, Thailand. Electronic address: kraingkrai.p@msu.ac.th.

PMID: 37922797
DOI: 10.1016/j.foodchem.2023.137879

Abstract

Iron-natural phenolic microparticles were developed as absorbents for dispersive micro solid phase extraction (D-μSPE) synergistic with hydrophobic ionic liquid (IL) for dispersive liquid-liquid microextraction (DLLME) to enrich tetracycline residues, including tetracycline, doxycycline, oxytetracycline and chlortetracycline. In situ iron microparticles synthesized from betel nut natural reagent were employed as an adsorbent for D-μSPE. The hydrophobic IL [Hmim][PF₆] was synergistically utilized as an extraction solvent to extract and accumulate adsorbents bound with tetracyclines before quantitation by HPLC-UV. The synergistic combination of DLLME with D-μSPE provided excellent extraction recovery compared with individual extraction. The developed method was successfully applied to enrich and determine tetracycline residues in honey samples, with recoveries ranging from 80.0 to 121.5% and providing high enrichment factors ranging from 61 to 197. This alternative method is simple and rapid, with high extraction efficiency and a high enrichment factor and is also environmentally friendly for the analysis of tetracyclines.

Keywords: Betel nut; Coextraction; Dispersive liquid–liquid microextraction; Dispersive micro solid phase extraction; Green extraction method; Natural reagent.

MeSH terms

Anti-Bacterial Agents / analysis
Chromatography, High Pressure Liquid / methods
Honey* / analysis
Ionic Liquids* / chemistry
Limit of Detection
Liquid Phase Microextraction* / methods
Phenols / analysis
Tetracycline / analysis
Tetracyclines / analysis

Substances

Ionic Liquids
Tetracycline
Anti-Bacterial Agents
Tetracyclines
Phenols