Recently, surface plasmon resonance (SPR) biosensors have been widely used in environmental monitoring, food contamination detection and diagnosing medical conditions due to their superior sensitivity, label-free detection and rapid analysis speed. This paper briefly elaborates on the development history of SPR technology and introduces SPR signal sensing principles. A summary of recent applications of SPR sensors in different fields is highlighted, including their figures of merit and limitations. Finally, the personal perspectives and future development trends about sensor preparation and design are discussed in detail, which may be critical for improving the performance of SPR sensors.