Exosomes from magnetic particles-primed mesenchymal stem cells enhance neural differentiation of PC12 cells

Yong Xie; Xiaoyan Wang; Zhonghui Wang; Jianyang Feng; Dongsheng Li

doi:10.1016/j.heliyon.2023.e21075

Exosomes from magnetic particles-primed mesenchymal stem cells enhance neural differentiation of PC12 cells

Heliyon. 2023 Oct 16;9(10):e21075. doi: 10.1016/j.heliyon.2023.e21075. eCollection 2023 Oct.

Authors

Yong Xie^{1

2}, Xiaoyan Wang³, Zhonghui Wang⁴, Jianyang Feng⁵, Dongsheng Li⁶

Affiliations

¹ Department of Obstetrics and Gynecology, The First People's Hospital of Foshan, Foshan 528000, China.
² Department of Obstetrics and Gynecology, Leping Affiliated Hospital of the First People's Hospital of Foshan, Foshan 528100, China.
³ Department of Gynecology, Shanxi Province Cancer Hospital, Shanxi Hospital Affiliated to Cancer Hospital, Chinese Academy of Medical Sciences, Cancer Hospital Affiliated to Shanxi Medical University, Taiyuan 030013, China.
⁴ Department of Hepatobiliary Surgery, The First People's Hospital of Foshan, Foshan 528000, China.
⁵ Department of Obstetrics and Gynecology, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510000, China.
⁶ DeJa Lab, VetCell Biotech Limited, Foshan 528225, China.

Abstract

This study aimed to investigate the effects of mesenchymal stem cell exosomes loaded with Fe₃O₄ magnetic particles (Fe₃O₄@ MSC-exo) on the survival and neural differentiation of PC12 cells. Exosomes were separated from Fe₃O₄ magnetic nanoparticles-primed umbilical cord mesenchymal stem cells condition medium by ultracentrifugation and characterized by transmission electron microscopy, flow nano analysis, and western blotting. PC12 cells were treated with culture medium containing exosomes. The effects of Fe₃O₄@ MSC-exo on PC12 cell proliferation, migration, and neural differentiation were analyzed using CCK-8 assay, transwell migration assay, RT-qPCR, and immunofluorescence, respectively. Additionally, miRNA sequencing was performed on Fe₃O₄@ MSC-exo, followed by bioinformatic analysis of the results. We found that Fe₃O₄@ MSC-exo can promote PC12 cell proliferation, migration, and neural differentiation. According to the sequencing results, there were a total of 43 differentially expressed miRNAs. The present study indicated that Fe₃O₄@ MSC-exo might enhance nerve cell function, laying the foundation for targeted therapy of nerve injury.

Keywords: Exosomes; Magnetic particles; Mesenchymal stem cells; Nerve injury.