Analysis of microbiota reveals the underlying mechanism of PHF11 in the development of Enterococcus-regulated endometriotic cysts

Jingjing Hu; Guanjian Li; Miaomiao Huang; Qunshan Shen; Hao Gu; Nairui Xue; Junqiang Zhang; Xiaofeng Xu; Wenjuan Yang; Aying Xing; Xiao Wu; Ying Wang; Rong Wu; Chuan Xu; Yuqian Li; Yuan Fang; Yunxia Cao

doi:10.1016/j.isci.2023.108158

Analysis of microbiota reveals the underlying mechanism of PHF11 in the development of Enterococcus-regulated endometriotic cysts

iScience. 2023 Oct 7;26(11):108158. doi: 10.1016/j.isci.2023.108158. eCollection 2023 Nov 17.

Authors

Jingjing Hu^{1

2

3}, Guanjian Li^{1

2

3}, Miaomiao Huang^{1

2

3}, Qunshan Shen^{1

2

3}, Hao Gu⁴, Nairui Xue^{1

2

3}, Junqiang Zhang^{1

2

5}, Xiaofeng Xu^{1

2

5}, Wenjuan Yang^{1

2

6}, Aying Xing^{1

2

6}, Xiao Wu^{1

2

6}, Ying Wang^{1

2

6}, Rong Wu^{1

2

6}, Chuan Xu^{1

2

6}, Yuqian Li^{1

2

7}, Yuan Fang^{1

2

7}, Yunxia Cao^{1

2

3}

Affiliations

¹ Department of Obstetrics and Gynecology, The First Affiliated Hospital of Anhui Medical University, No 218 Jixi Road, Hefei, Anhui 230022, China.
² NHC Key Laboratory of Study on Abnormal Gametes and Reproductive Tract (Anhui Medical University), No 81 Meishan Road, Hefei, Anhui 230032, China.
³ Key Laboratory of Population Health Across Life Cycle (Anhui Medical University), Ministry of Education of the People's Republic of China, No 81 Meishan Road, Hefei, Anhui 230032, China.
⁴ School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui 230022, China.
⁵ Engineering Research Center of Biopreservation and Artifical Organs, Ministry of Education, No 81 Meishan Road, Hefei, Anhui 230032, China.
⁶ Anhui Province Key Laboratory of Reproductive Health and Genetics, No 81 Meishan Road, Hefei, Anhui 230032, China.
⁷ Biopreservation and Artificial Organs, Anhui Provincial Engineering Research Center, Anhui Medical University, No 81 Meishan Road, Hefei, Anhui 230032, China.

Abstract

Endometriosis (EMS) is a prevalent disease and the etiologies has not uniform. Microbiota is associated with human diseases. To delve into the relationship between EMS and microbiota, Ectopic (EM) and eutopic (EU) endometrial tissues, pharyngeal swabs, and stools were collected from EMS patients. The microbiota composition of EM and EU partially overlapped, with similar taxon numbers and diversity, but the richness levels were significantly different. A comparison of intestinal microbes in healthy individuals (FN) and EMS patients (FE) revealed that the richness of Enterococcus, Pseudomonas, Haemophilus, and Neisseria was enhanced in FE. In addition, Enterococcus-induced mice (EFA) presented with a higher degree of lesion infiltration and a wider distribution of lesions. Proteomic analysis revealed the expression of plant homeodomain finger 11 (PHF11) was notably downregulated in EFA. And the downregulated expression of PHF11 was accompanied by the upregulated expression of interleukin 8 (IL-8). Our findings suggest a potential regulatory mechanism for PHF11 in EMS development.

Keywords: Biological sciences; Microbiology; Plant biology.