DNA Barcode library of the endemic-rich avifauna of the oceanic islands of the Gulf of Guinea

Martim Melo; Rita Covas; Ricardo Faustino de Lima; Octávio Veiga da Horta; Ceciliano do Bom Jesus; Martim Barros da Veiga; Seduney Samba; Ricardo Fonseca; Gabriel Cabinda; Lionel Viegas; Teresa L Silva; Vanessa A Mata; Pedro Beja; Sónia Ferreira

doi:10.3897/BDJ.11.e110428

DNA Barcode library of the endemic-rich avifauna of the oceanic islands of the Gulf of Guinea

Biodivers Data J. 2023 Oct 23:11:e110428. doi: 10.3897/BDJ.11.e110428. eCollection 2023.

Authors

Martim Melo^{1

2

3

4}, Rita Covas^{1

2

4}, Ricardo Faustino de Lima^{5

6

7

8}, Octávio Veiga da Horta⁹, Ceciliano do Bom Jesus¹⁰, Martim Barros da Veiga⁹, Seduney Samba⁹, Ricardo Fonseca⁹, Gabriel Cabinda⁹, Lionel Viegas¹¹, Teresa L Silva^{1

2}, Vanessa A Mata^{1

2}, Pedro Beja^{1

2

12}, Sónia Ferreira^{1

2}

Affiliations

¹ CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, InBIO Laboratório Associado, Campus de Vairao, Universidade do Porto, 4485-661 Vairao, Vila do Conde, Portugal CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, InBIO Laboratório Associado, Campus de Vairao, Universidade do Porto, 4485-661 Vairao Vila do Conde Portugal.
² BIOPOLIS Program in Genomics, Biodiversity and Land Planning, CIBIO, Campus de Vairao, 4485-661 Vairao, Vila do Conde, Portugal BIOPOLIS Program in Genomics, Biodiversity and Land Planning, CIBIO, Campus de Vairao, 4485-661 Vairao Vila do Conde Portugal.
³ MHNC-UP, Museu de História Natural e da Ciência da Universidade do Porto, Porto, Portugal MHNC-UP, Museu de História Natural e da Ciência da Universidade do Porto Porto Portugal.
⁴ DST/NRF Centre of Excellence, FitzPatrick Institute, University of Cape Town, Rondebosch, South Africa DST/NRF Centre of Excellence, FitzPatrick Institute, University of Cape Town Rondebosch South Africa.
⁵ Gulf of Guinea Biodiversity Centre, São Tomé, São Tomé and Príncipe Gulf of Guinea Biodiversity Centre São Tomé São Tomé and Príncipe.
⁶ Centre for Ecology, Evolution and Environmental Changes, Faculdade de Ciências da Universidade de Lisboa, Lisboa, Portugal Centre for Ecology, Evolution and Environmental Changes, Faculdade de Ciências da Universidade de Lisboa Lisboa Portugal.
⁷ Departamento de Biologia Animal, Faculdade de Ciências, Universidade de Lisboa, Lisboa, Portugal Departamento de Biologia Animal, Faculdade de Ciências, Universidade de Lisboa Lisboa Portugal.
⁸ CHANGE - Global Change and Sustainability Institute, Faculdade de Ciências, Universidade de Lisboa, Lisboa, Portugal CHANGE - Global Change and Sustainability Institute, Faculdade de Ciências, Universidade de Lisboa Lisboa Portugal.
⁹ Associação Monte Pico, Monte Café, São Tomé, São Tomé and Príncipe Associação Monte Pico, Monte Café São Tomé São Tomé and Príncipe.
¹⁰ Parque Natural do Obô - Príncipe, Porto Real, Príncipe, São Tomé and Príncipe Parque Natural do Obô - Príncipe, Porto Real Príncipe São Tomé and Príncipe.
¹¹ Parque Nacional do Obô - São Tomé, São Tomé, São Tomé and Príncipe Parque Nacional do Obô - São Tomé São Tomé São Tomé and Príncipe.
¹² CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, InBIO Laboratório Associado, Instituto Superior de Agronomia, Universidade de Lisboa, Lisboa, Portugal CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, InBIO Laboratório Associado, Instituto Superior de Agronomia, Universidade de Lisboa Lisboa Portugal.

Abstract

Background: The BioSTP: DNA Barcoding of endemic birds from oceanic islands of the Gulf of Guinea dataset contains records of 155 bird specimens belonging to 56 species in 23 families, representing over 80% of the diversity of the breeding landbird community. All specimens were collected on Príncipe, São Tomé and Annobón Islands between 2002 and 2021 and morphologically identified to species or subspecies level by qualified ornithologists. The dataset includes all endemic species and 3/4 of the extant endemic subspecies of the islands. This dataset is the second release by BioSTP and it greatly increases the knowledge on the DNA barcodes of Gulf of Guinea birds. All DNA extractions are deposited at Associação BIOPOLIS - CIBIO, Research Center in Biodiversity and Genetic Resources.

New information: The dataset includes DNA barcodes for all 29 endemic bird species and for 11 of the 15 extant endemic bird subspecies from the oceanic islands of the Gulf of Guinea. This is the first major DNA barcode set of African birds. The three endemic subspecies of Crithagrarufobrunnea, an island endemic with three allopatric populations within the Archipelago, are also represented. Additionally, we obtained DNA barcodes for 16 of the 21 non-endemic landbirds and for one vagrant (Sylviacommunis). In total, forty-one taxa were new additions to the Barcode of Life Data System (BOLD), with another 11 corresponding to under-represented taxa in BOLD. Furthermore, the submitted sequences were found to cluster in 55 Barcode Index Numbers (BINs), 37 of which were new to BOLD. All specimens have their DNA barcodes publicly accessible through BOLD online database and GenBank.

Keywords: Annobón; COI; DNA barcode; Príncipe; São Tomé; aves.

Martim Melo, Rita Covas, Ricardo Faustino de Lima, Octávio Veiga da Horta, Ceciliano do Bom Jesus, Martim Barros da Veiga, Seduney Samba, Ricardo Fonseca, Gabriel Cabinda, Lionel Viegas, Teresa L Silva, Vanessa A. Mata, Pedro Beja, Sónia Ferreira.

Grants and funding

The present study was funded by the project TROPIBIO NORTE-01-0145-FEDER-000046, supported by Norte Portugal Regional Operational Programme (NORTE2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF) and was carried out in collaboration with BirdLife International, in the framework of the partnership agreement between BirdLife International and BIOPOLIS association to promote informed, evidence-based biodiversity conservation action in Sao Tomé and Principe, which is funded by the European Union through the ‘Landscape Management in Sao Tomé and Principe’ project (ENV/2020/420-182)