3D digital polarization-holographic wavelet histology in determining the duration of mechanical damage to the myocardium

J Biophotonics. 2024 Mar;17(3):e202300372. doi: 10.1002/jbio.202300372. Epub 2024 Jan 10.

Abstract

We aimed developing and experimentally validating methods for 3D scale-selective polarimetry of multiply scattered fields in diffuse myocardium layers for mechanical myocardial injury prescription histological differential diagnostics. We used the synthesis of diffuse object field polarization-interference registration and polarization-inhomogeneous field digital holographic reconstruction and layer-by-layer complex amplitudes distributions The method for selection single and diffuse object field multiply scattered components polarization maps is proposed. The conditions for eliminating the distorting influence of a depolarized background high level are found. On the basis of еру object field single scattered component polarization maps a large-scale selective wavelet analysis the criteria (markers) for mechanical myocardial injury different prescription diagnosis was determinate. Excellent accuracy mechanical injury myocardium necrotic changes with different duration using polarization-interference wavelet differentiation were achieved.

Keywords: holography; interference; mechanical injury; myocardium; optical anisotropy; polarization; statistical moments; wavelet analysis.

MeSH terms

  • Heart* / diagnostic imaging
  • Myocardium* / pathology