Purification, structural characteristics and anti-atherosclerosis activity of a novel green tea polysaccharide

Chao Zheng; Shun Chen; Yuan-Yuan Deng; Xin-Ping Qian; Ying-Ying Chen; Cheng-Zhi Hong; Ya-Fan Zeng; Qiang-Ming Li; Li-Hua Pan; Jian-Ping Luo; Xue-Ying Li; Xue-Qiang Zha

doi:10.1016/j.ijbiomac.2023.127705

Purification, structural characteristics and anti-atherosclerosis activity of a novel green tea polysaccharide

Int J Biol Macromol. 2024 Jan;254(Pt 1):127705. doi: 10.1016/j.ijbiomac.2023.127705. Epub 2023 Oct 31.

Authors

Affiliations

¹ Engineering Research Centre of Bioprocess of Ministry of Education, School of Food and Biological Engineering, Hefei University of Technology, No. 193 Tunxi Road, Hefei 230009, People's Republic of China; School of Food and Biological Engineering, Hefei University of Technology, No. 193 Tunxi Road, Hefei 230009, People's Republic of China.
² Sericultural and Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510610, People's Republic of China.
³ Engineering Research Centre of Bioprocess of Ministry of Education, School of Food and Biological Engineering, Hefei University of Technology, No. 193 Tunxi Road, Hefei 230009, People's Republic of China; School of Food and Biological Engineering, Hefei University of Technology, No. 193 Tunxi Road, Hefei 230009, People's Republic of China. Electronic address: lwying17@163.com.
⁴ Engineering Research Centre of Bioprocess of Ministry of Education, School of Food and Biological Engineering, Hefei University of Technology, No. 193 Tunxi Road, Hefei 230009, People's Republic of China; School of Food and Biological Engineering, Hefei University of Technology, No. 193 Tunxi Road, Hefei 230009, People's Republic of China. Electronic address: zhaxueqiang@hfut.edu.cn.

PMID: 37913884
DOI: 10.1016/j.ijbiomac.2023.127705

Abstract

A new homogeneous polysaccharide (TPS3A) was isolated and purified from Tianzhu Xianyue fried green tea by DEAE-52 cellulose and Sephacryl S-500 column chromatography. Structural characterization indicated that TPS3A mainly consisted of arabinose, galactose, galacturonic acid and rhamnose in a molar ratio of 5.84: 4.15: 2.06: 1, with an average molecular weight of 1.596 × 10⁴ kDa. The structure of TPS3A was characterized as a repeating unit consisting of 1,3-Galp, 1,4-Galp, 1,3,6-Galp, 1,3-Araf, 1,5-Araf, 1,2,4-Rhap and 1-GalpA, with two branches on the C6 of 1,3,6-Galp and C2 of 1,2,4-Rhap, respectively. To investigate the preventive effects of TPS3A on atherosclerosis, TPS3A was administered orally to ApoE-deficient (ApoE^-/-) mice. Results revealed that TPS3A intervention could effectively delay the atherosclerotic plaque progression, modulate dyslipidemia, and reduce the transformation of vascular smooth muscle cells (VSMCs) from contractile phenotype to synthetic phenotype by activating the expression of contractile marker alpha-smooth muscle actin (α-SMA) and inhibiting the expression of synthetic marker osteopontin (OPN) in high-fat diet-induced ApoE^-/- mice. Our findings suggested that TPS3A markedly alleviated atherosclerosis by regulating dyslipidemia and phenotypic transition of VSMCs, and might be used as a novel functional ingredient to promote cardiovascular health.

Keywords: Anti-atherosclerosis; Chemical structure; Green tea; Polysaccharide.

MeSH terms

Animals
Apolipoproteins E
Atherosclerosis* / drug therapy
Dyslipidemias*
Mice
Polysaccharides / analysis
Polysaccharides / pharmacology
Polysaccharides / therapeutic use
Tea

Substances

Tea
Polysaccharides
Apolipoproteins E