Multifunctional nanocoating for enhanced titanium implant osseointegration

Kun Li; Zhen Tang; Kuangyu Song; Nicholas G Fischer; Huihui Wang; Yunlin Guan; Yunyun Deng; Hao Cai; Sammer Ul Hassan; Zhou Ye; Ting Sang

doi:10.1016/j.colsurfb.2023.113604

Multifunctional nanocoating for enhanced titanium implant osseointegration

Colloids Surf B Biointerfaces. 2023 Dec:232:113604. doi: 10.1016/j.colsurfb.2023.113604. Epub 2023 Oct 18.

Authors

Kun Li¹, Zhen Tang¹, Kuangyu Song², Nicholas G Fischer³, Huihui Wang¹, Yunlin Guan¹, Yunyun Deng¹, Hao Cai¹, Sammer Ul Hassan⁴, Zhou Ye⁵, Ting Sang⁶

Affiliations

¹ School of Stomatology, Nanchang University, Nanchang, Jiangxi Province 330006, China; The Key Laboratory of Oral Biomedicine & Jiangxi Province Clinical Research Center for Oral Diseases, Nanchang, Jiangxi Province 330006, China.
² Department of Microbiology, School of Medicine, Nanchang University, Nanchang, Jiangxi Province 330006, China.
³ MDRCBB, Minnesota Dental Research Center for Biomaterials and Biomechanics, University of Minnesota, Minneapolis, MN 55455, United States.
⁴ Department of Mechanical Engineering, The University of Hong Kong, Hong Kong Special Administrative Region of China.
⁵ Applied Oral Sciences and Community Dental Care, Faculty of Dentistry, The University of Hong Kong, Hong Kong Special Administrative Region of China. Electronic address: zhouye22@hku.hk.
⁶ School of Stomatology, Nanchang University, Nanchang, Jiangxi Province 330006, China; The Key Laboratory of Oral Biomedicine & Jiangxi Province Clinical Research Center for Oral Diseases, Nanchang, Jiangxi Province 330006, China. Electronic address: sangting3@ncu.edu.cn.

PMID: 37913704
DOI: 10.1016/j.colsurfb.2023.113604

Abstract

Preventing bacterial infection and promoting osseointegration are essential for the long-term success of titanium (Ti) implants. In this study, we developed a multifunctional nanocoating on Ti mini-implants to simultaneously address these challenges. The nanocoating consists of self-assembled antimicrobial peptides GL13K and silver nanoparticles, referred to as Ag-GL. Our results showed that the Ag-GL coating did not alter the surface morphology of the mini-implants. Ag-GL coated mini-implants demonstrated a two orders of magnitude reduction in colony-forming unit (CFU) values compared to the noncoated eTi group, resulting in minimal inflammation and no apparent bone destruction in a bacterial infection in vivo model. When evaluating osseointegration properties, micro-CT analysis, histomorphometric analysis, and pull-out tests revealed that the Ag-GL coating significantly enhanced osseointegration and promoted new bone formation in vivo.

Keywords: Antimicrobial; Antimicrobial peptide; Implant infection; Osseointegration; Silver nanoparticle.

MeSH terms

Bacterial Infections*
Coated Materials, Biocompatible / chemistry
Coated Materials, Biocompatible / pharmacology
Humans
Metal Nanoparticles*
Osseointegration
Silver / chemistry
Silver / pharmacology
Surface Properties
Titanium / chemistry
Titanium / pharmacology

Substances

Titanium
Coated Materials, Biocompatible
Silver