Protocol for isolating single cells from human pancreatic cancer tissues and analyzing major immune cell populations using flow cytometry

Jinyuan Song; Junlei Zhang; Yongtao Ji; Jianghui Tang; Jianpeng Sheng; Tingbo Liang; Xueli Bai

doi:10.1016/j.xpro.2023.102679

Protocol for isolating single cells from human pancreatic cancer tissues and analyzing major immune cell populations using flow cytometry

STAR Protoc. 2023 Dec 15;4(4):102679. doi: 10.1016/j.xpro.2023.102679. Epub 2023 Oct 31.

Authors

Jinyuan Song¹, Junlei Zhang¹, Yongtao Ji¹, Jianghui Tang¹, Jianpeng Sheng², Tingbo Liang³, Xueli Bai⁴

Affiliations

¹ Department of Hepatobiliary and Pancreatic Surgery, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310002, China; Zhejiang Provincial Key Laboratory of Pancreatic Disease, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310002, China; Zhejiang University Cancer Center, Zhejiang University, Hangzhou 310002, China.
² Department of Hepatobiliary and Pancreatic Surgery, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310002, China; Zhejiang Provincial Key Laboratory of Pancreatic Disease, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310002, China; Zhejiang University Cancer Center, Zhejiang University, Hangzhou 310002, China. Electronic address: shengjp@zju.edu.cn.
³ Department of Hepatobiliary and Pancreatic Surgery, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310002, China; Zhejiang Provincial Key Laboratory of Pancreatic Disease, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310002, China; Zhejiang University Cancer Center, Zhejiang University, Hangzhou 310002, China. Electronic address: liangtingbo@zju.edu.cn.
⁴ Department of Hepatobiliary and Pancreatic Surgery, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310002, China; Zhejiang Provincial Key Laboratory of Pancreatic Disease, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310002, China; Zhejiang University Cancer Center, Zhejiang University, Hangzhou 310002, China. Electronic address: shirleybai@zju.edu.cn.

Abstract

Here, we present a protocol for collecting, dissociating, isolating, staining, and analyzing immune cells from pancreatic cancer tissues for flow cytometry. The isolated cells can also be used for single-cell RNA sequencing and other related procedures. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2023).¹.

Keywords: Cancer; Cell Biology; Cell Isolation; Clinical Protocol; Flow Cytometry; Health Sciences; Single Cell.

MeSH terms

Flow Cytometry
Humans
Pancreatic Neoplasms* / genetics
Staining and Labeling