A Novel Monochromatic Light to Detect Target Specimens Within Endoscopic Ultrasonography-guided Fine-needle Aspiration Biopsy Samples

Kazuya Matsumoto; Masaru Ueki; Kunio Ichino; Takeshi Tsuka; Kazutake Uehara; Yohei Takeda; Takumi Onoyama; Soichiro Kawata; Hiroki Kurumi; Hiroki Koda; Taro Yamashita; Yoshiharu Okamoto; Naoyuki Yamaguchi; Shigeo Sasaki; Hajime Isomoto

doi:10.21873/invivo.13348

A Novel Monochromatic Light to Detect Target Specimens Within Endoscopic Ultrasonography-guided Fine-needle Aspiration Biopsy Samples

In Vivo. 2023 Nov-Dec;37(6):2433-2436. doi: 10.21873/invivo.13348.

Authors

Kazuya Matsumoto^{1

2}, Masaru Ueki³, Kunio Ichino⁴, Takeshi Tsuka⁵, Kazutake Uehara³, Yohei Takeda⁶, Takumi Onoyama⁶, Soichiro Kawata⁶, Hiroki Kurumi⁶, Hiroki Koda⁶, Taro Yamashita⁶, Yoshiharu Okamoto⁵, Naoyuki Yamaguchi⁷, Shigeo Sasaki⁸, Hajime Isomoto⁶

Affiliations

¹ Division of Medicine and Clinical Science, Department of Multidisciplinary Internal Medicine, Faculty of Medicine, Tottori University, Yonago, Japan; matsumotokazuya@tottori-u.ac.jp.
² Irisawa Medical Clinic, Matsue, Japan.
³ Advanced Medicine, Innovation and Clinical Research Center, Tottori University Hospital, Yonago, Japan.
⁴ Department of Information and Electronics, Graduate School of Engineering, Tottori University, Tottori, Japan.
⁵ Department of Veterinary Clinical Medicine, School of Veterinary Medicine, Tottori University, Tottori, Japan.
⁶ Division of Medicine and Clinical Science, Department of Multidisciplinary Internal Medicine, Faculty of Medicine, Tottori University, Yonago, Japan.
⁷ Department of Gastroenterology and Hepatology, Nagasaki University Graduate School of Biological Sciences, Nagasaki, Japan.
⁸ Organization for Research Initiative and Promotion, Tottori University, Tottori, Japan.

Abstract

Background/aim: Endoscopic ultrasonography (EUS)-guided fine-needle aspiration biopsy (EUS-FNB) enhances the diagnostic capabilities of EUS by providing additional pathological samples. However, detecting the target specimens within the collected samples can be challenging. The aim of this study was to determine the optimal wavelength of light for detection of target specimens within EUS-FNB samples in an animal experiment.

Materials and methods: EUS-FNB pancreatic tissue samples were collected from a male beagle (weight, 10 kg), and the samples were illuminated with monochromatic light ranging from 430 to 700 nm in 5-nm intervals. The intensities of the target specimen and blood samples were analyzed using the densitometry of the images obtained through irradiation.

Results: We found that transmitted monochromatic light of 605 nm most vividly enhanced the contrast between the target specimens and blood in the samples in the impression of appearance.

Conclusion: Thus, microscopical observations under transmitted light of 605 nm are optimal for target tissue identification within EUS-FNB samples.

Keywords: Endoscopic ultrasound-guided fine needle aspiration; light microscopy; pancreas; rapid on-site evaluation.

MeSH terms

Animals
Dogs
Endoscopic Ultrasound-Guided Fine Needle Aspiration / methods
Endoscopy
Endosonography*
Male
Pancreatic Neoplasms* / pathology