Heteronuclear and homonuclear radio-frequency-driven recoupling

Evgeny Nimerovsky; Kai Xue; Kumar Tekwani Movellan; Loren B Andreas

doi:10.5194/mr-2-343-2021

Heteronuclear and homonuclear radio-frequency-driven recoupling

Magn Reson (Gott). 2021 May 28;2(1):343-353. doi: 10.5194/mr-2-343-2021. eCollection 2021.

Authors

Evgeny Nimerovsky¹, Kai Xue¹, Kumar Tekwani Movellan¹, Loren B Andreas¹

Affiliation

¹ Department of NMR-based Structural Biology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, Göttingen, Germany.

Abstract

The radio-frequency-driven recoupling (RFDR) pulse sequence is used in magic-angle spinning (MAS) NMR to recouple homonuclear dipolar interactions. Here we show simultaneous recoupling of both the heteronuclear and homonuclear dipolar interactions by applying RFDR pulses on two channels. We demonstrate the method, called HETeronuclear RFDR (HET-RFDR), on microcrystalline SH3 samples at 10 and 55.555 kHz MAS. Numerical simulations of both HET-RFDR and standard RFDR sequences allow for better understanding of the influence of offsets and paths of magnetization transfers for both HET-RFDR and RFDR experiments, as well as the crucial role of XY phase cycling.