Isolation and identification of antagonistic Bacillus amyloliquefaciens HSE-12 and its effects on peanut growth and rhizosphere microbial community

Huying Li; Chaohui Li; Xin Song; Jintai Li; Pengcheng Zhang; Fengxia Sun; Zhigang Geng; Xunli Liu

doi:10.3389/fmicb.2023.1274346

Isolation and identification of antagonistic Bacillus amyloliquefaciens HSE-12 and its effects on peanut growth and rhizosphere microbial community

Front Microbiol. 2023 Oct 12:14:1274346. doi: 10.3389/fmicb.2023.1274346. eCollection 2023.

Authors

Huying Li^{1

2}, Chaohui Li¹, Xin Song¹, Jintai Li¹, Pengcheng Zhang¹, Fengxia Sun^{1

3}, Zhigang Geng⁴, Xunli Liu^{1

3}

Affiliations

¹ College of Forestry, Shandong Agricultural University, Taian, China.
² School of Nursing, Zibo Vocational Institute, Zibo, China.
³ Key Laboratory of National Forestry and Grassland Administration on Silviculture of the Lower Yellow River, Shandong Agricultural University, Taian, China.
⁴ Ministry of Agriculture Key Laboratory of Seaweed Fertilizers, Qingdao, China.

Abstract

The HSE-12 strain isolated from peanut rhizosphere soil was identified as Bacillus amyloliquefaciens by observation of phenotypic characteristics, physiological and biochemical tests, 16S rDNA and gyrB gene sequencing. In vitro experiments showed that the strain possessed biocontrol activity against a variety of pathogens including Sclerotium rolfsii. The strain has the ability to produce hydrolytic enzymes, as well as volatile organic compounds with antagonistic and probiotic effects such as ethyleneglycol and 2,3-butanediol. In addition, HSE-12 showed potassium solubilizing (10.54 ± 0.19 mg/L), phosphorus solubilization (168.34 ± 8.06 mg/L) and nitrogen fixation (17.35 ± 2.34 mg/g) abilities, and was able to secrete siderophores [(Ar-A)/Ar × 100%: 56%] which promoted plant growth. After inoculating peanut with HSE-12, the available phosphorus content in rhizosphere soil increased by 27%, urease activity increased by 43%, catalase activity increased by 70% and sucrase activity increased by 50% (p < 0.05). The dry weight, fresh weight and the height of the first pair of lateral branches of peanuts increased by 24.7, 41.9, and 36.4%, respectively, compared with uninoculated peanuts. In addition, compared with the blank control, it increased the diversity and richness of peanut rhizosphere bacteria and changed the community structure of bacteria and fungi. The relative abundance of beneficial microorganisms such as Sphingomonas, Arthrobacter, RB41, and Micromonospora in rhizosphere soil was increased, while the relative abundance of pathogenic microorganisms such as Aspergillus, Neocosmospora, and Rhizoctonia was decreased.

Keywords: Bacillus amyloliquefaciens; biocontrol; microbial community; plant growth-promoting rhizobacteria; volatile compounds.

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This research was funded by the West Coast Science and Technology Foundation of Qingdao (2019–23).