Genome-wide linkage mapping of root system architecture-related traits in common wheat (Triticum aestivum L.)

Yirong Jin; Yamei Wang; Jindong Liu; Fuyan Wang; Xiaodong Qiu; Peng Liu

doi:10.3389/fpls.2023.1274392

Genome-wide linkage mapping of root system architecture-related traits in common wheat (Triticum aestivum L.)

Front Plant Sci. 2023 Oct 13:14:1274392. doi: 10.3389/fpls.2023.1274392. eCollection 2023.

Authors

Yirong Jin¹, Yamei Wang², Jindong Liu³, Fuyan Wang¹, Xiaodong Qiu⁴, Peng Liu¹

Affiliations

¹ Wheat Research Institute, Dezhou Academy of Agricultural Sciences, Dezhou, China.
² School of Agriculture, Sun Yat-sen University, Shenzhen, China.
³ Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
⁴ Department of Science and Technology of Shandong Province, Jinan, China.

Abstract

Identifying loci for root system architecture (RSA) traits and developing available markers are crucial for wheat breeding. In this study, RSA-related traits, including total root length (TRL), total root area (TRA), and number of root tips (NRT), were evaluated in the Doumai/Shi4185 recombinant inbred line (RIL) population under hydroponics. In addition, both the RILs and parents were genotyped using the wheat 90K single-nucleotide polymorphism (SNP) array. In total, two quantitative trait loci (QTLs) each for TRL (QTRL.caas-4A.1 and QTRL.caas-4A.2), TRA (QTRA.caas-4A and QTRA.caas-4D), and NRT (QNRT.caas-5B and QNRT.caas-5D) were identified and each explaining 5.94%-9.47%, 6.85%-7.10%, and 5.91%-10.16% phenotypic variances, respectively. Among these, QTRL.caas-4A.1 and QTRA.caas-4A overlapped with previous reports, while QTRL.caas-4A.2, QTRA.caas-4D, QNRT.caas-5B, and QNRT.caas-5D were novel. The favorable alleles of QTRL.caas-4A.1, QTRA.caas-4A, and QTRA.caas-5B were contributed by Doumai, whereas the favorable alleles of QTRL.caas-4A.2, QTRA.caas-4D, and QTRA.caas-5D originated from Shi 4185. Additionally, two competitive allele-specific PCR (KASP) markers, Kasp_4A_RL (QTRA.caas-4A) and Kasp_5D_RT (QNRT.caas-5D), were developed and validated in 165 wheat accessions. This study provides new loci and available KASP markers, accelerating wheat breeding for higher yields.

Keywords: bread wheat; marker-assisted selection; quantitative trait locus (QTL); root system architecture (RSA); single-nucleotide polymorphism (SNP).

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This study was financially supported by the Key Project of Shandong Natural Science Foundation (ZR2020KC042); Shandong Agricultural Seeds Engineering Project (2021LZGC013); and Agricultural Science and Technology Innovation Project of Shandong (2020CXGC01080517).