Water deficit and potassium affect carbon isotope composition in cassava bulk leaf material and extracted carbohydrates

Jonas Van Laere; Roel Merckx; Rebecca Hood-Nowotny; Gerd Dercon

doi:10.3389/fpls.2023.1222558

Water deficit and potassium affect carbon isotope composition in cassava bulk leaf material and extracted carbohydrates

Front Plant Sci. 2023 Oct 13:14:1222558. doi: 10.3389/fpls.2023.1222558. eCollection 2023.

Authors

Jonas Van Laere^{1

2

3}, Roel Merckx², Rebecca Hood-Nowotny³, Gerd Dercon¹

Affiliations

¹ Soil and Water Management & Crop Nutrition Laboratory, Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Vienna, Austria.
² Division of Soil and Water Management, Department of Earth and Environmental Sciences, KU Leuven, Heverlee, Belgium.
³ Institute of Soil Research, Department of Forest and Soil Sciences, University of Natural Resources and Life Sciences Vienna, Vienna, Austria.

Abstract

Cassava (Manihot esculenta Crantz) is an important root crop, which despite its drought tolerance suffers considerable yield losses under water deficit. One strategy to increase crop yields under water deficit is improving the crop's transpiration efficiency, which could be achieved by variety selection and potassium application. We assessed carbon isotope composition in bulk leaf material and extracted carbohydrates (soluble sugar, starch, and cellulose) of selected leaves one month after inducing water deficit to estimate transpiration efficiency and storage root biomass under varying conditions in a greenhouse experiment. A local and improved variety were grown in sand, supplied with nutrient solution with two potassium levels (1.44 vs. 0.04 mM K⁺) and were subjected to water deficit five months after planting. Potassium application and selection of the improved variety both increased transpiration efficiency of the roots with 58% and 85% respectively. Only in the improved variety were ¹³C ratios affected by potassium application (up to - 1.8‰ in δ¹³C of soluble sugar) and water deficit (up to + 0.6‰ in δ¹³C of starch and soluble sugar). These data revealed a shift in substrate away from transitory starch for cellulose synthesis in young leaves of the improved variety under potassium deficit. Bulk δ¹³C of leaves that had fully developed prior to water deficit were the best proxies for storage root biomass (r = - 0.62, r = - 0.70) and transpiration efficiency (r = - 0.68, r = - 0.58) for the local and improved variety respectively, making laborious extractions redundant. Results obtained from the youngest fully developed leaf, commonly used as a diagnostic leaf, were complicated by remobilized assimilates in the improved variety, making them less suitable for carbon isotope analysis. This study highlights the potential of carbon isotope composition to assess transpiration efficiency and yield, depending on the chosen sampling strategy as well as to unravel carbon allocation processes.

Keywords: Manihot esculenta Crantz; carbon isotope discrimination; cellulose; drought; nutrient stress; soluble sugar; starch; water use efficiency.

Grants and funding

This work was supported by the Belgian government through the Consortium for Improving Agriculture-based Livelihoods in Central Africa (CIALCA, www.cialca.org).