Acoustic droplet ejection facilitates cell-based high-throughput screenings using natural products

Thomas A Mackenzie; José R Tormo; Bastien Cautain; Germán Martínez; Isabel Sánchez; Olga Genilloud; Francisca Vicente; Maria C Ramos

doi:10.1016/j.slast.2023.10.003

Acoustic droplet ejection facilitates cell-based high-throughput screenings using natural products

SLAS Technol. 2023 Oct 26:S2472-6303(23)00062-6. doi: 10.1016/j.slast.2023.10.003. Online ahead of print.

Authors

Thomas A Mackenzie¹, José R Tormo¹, Bastien Cautain¹, Germán Martínez¹, Isabel Sánchez¹, Olga Genilloud¹, Francisca Vicente¹, Maria C Ramos²

Affiliations

¹ Fundación MEDINA, Av. Conocimiento 34, Parque Tecnológico de la Salud, 18016 Granada, Spain.
² Fundación MEDINA, Av. Conocimiento 34, Parque Tecnológico de la Salud, 18016 Granada, Spain. Electronic address: carmen.ramos@medinaandalucia.es.

PMID: 37898289
DOI: 10.1016/j.slast.2023.10.003

Abstract

Natural Products (NPs) are one of the main sources for drug discovery. Many clinical drugs are NPs or NP-inspired compounds, and recently discovered New Chemical Entities (NCEs) of NPs are emerging as promising new drugs. High-Throughput Screening (HTS) of large sample sets or libraries has grown to be vital for the drug discovery field. Industrial-scale HTS of NP libraries can be limited due to the difficulties entailed in working with tiny extract volumes and the variability in viscosity of NP extracts. For these reasons, the implementation of new technologies to miniaturize different reagent volumes grows to be fundamental. Since Acoustic Droplet Ejection (ADE) emerged as a helpful tool in HTS campaigns for the transference of compound libraries. The aim of this work was to test the effectiveness of ADE for the dispensation of NP extract libraries in cell-based HTS assays.

Keywords: Acoustic droplet ejection (ADE); High throughput screening (HTS); Natural products (NP).