This study presents an innovative, efficient, environmentally friendly and rapid mechanical inclusion extraction (MIE) method for active ingredients in functional food. 2-hydroxypropyl-beta-cyclodextrin was used as the inclusion reagent, and water was used as the extraction solvent in MIE. The experimental parameters affecting the extraction efficiency of the target compounds were systematically investigated using single-factor experiments and surface response methodology optimization. The method showed satisfactory linearity (coefficient of determination > 0.991), precision (0.02 % to 4.89 %), limit of detection (1.1-11.3 ng/mL), and recoveries of 80.4-108.7 % and 86.3-112.3 % at spiked concentration levels of 1 and 5 μg/mL, respectively. Consequently, the MIE method provided a novel green alternative and extended its applications for the simultaneous extraction of hydrophobic and hydrophilic compounds from functional food.
Keywords: Flavonoids; Functional food; Mechanical inclusion extraction; Terpenoids; Ultrahigh-performance liquid chromatography; β-Cyclodextrin.
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