Identification and Characterization of a Novel Hypovirus from the Phytopathogenic Fungus Botryosphaeria dothidea

Yongqi Wen; Jinyue Qu; Honglin Zhang; Yi Yang; Rui Huang; Jili Deng; Jiayu Zhang; Yanping Xiao; Jiali Li; Meixin Zhang; Guoping Wang; Lifeng Zhai

doi:10.3390/v15102059

Identification and Characterization of a Novel Hypovirus from the Phytopathogenic Fungus Botryosphaeria dothidea

Viruses. 2023 Oct 7;15(10):2059. doi: 10.3390/v15102059.

Authors

Yongqi Wen¹, Jinyue Qu¹, Honglin Zhang¹, Yi Yang¹, Rui Huang¹, Jili Deng¹, Jiayu Zhang¹, Yanping Xiao¹, Jiali Li¹, Meixin Zhang¹, Guoping Wang², Lifeng Zhai¹

Affiliations

¹ College of Life Science and Biotechnology, Yangtze Normal University, Chongqing 408100, China.
² Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.

Abstract

Many mycoviruses have been accurately and successfully identified in plant pathogenic fungus Botryosphaeria dothidea. This study discovered three mycoviruses from a B. dothidea strain SXD111 using high-throughput sequencing technology. A novel hypovirus was tentatively named Botryosphaeria dothidea hypovirus 1 (BdHV1/SXD111). The other two were known viruses, which we named Botryosphaeria dothidea polymycovirus 1 strain SXD111 (BdPmV1/SXD111) and Botryosphaeria dothidea partitivirus 1 strain SXD111 (BdPV1/SXD111). The genome of BdHV1/SXD111 is 11,128 nucleotides long, excluding the poly (A) tail. A papain-like cysteine protease (Pro), a UDP-glucose/sterol glucosyltransferase (UGT), an RNA-dependent RNA polyprotein (RdRp), and a helicase (Hel) were detected in the polyprotein of BdHV1/SXD111. Phylogenetic analysis showed that BdHV1/SXD111 was clustered with betahypovirus and separated from members of the other genera in the family Hypoviridae. The BdPmV1/SXD111 genome comprised five dsRNA segments with 2396, 2232, 1967, 1131, and 1060 bp lengths. Additionally, BdPV1/SXD111 harbored three dsRNA segments with 1823, 1623, and 557 bp lengths. Furthermore, the smallest dsRNA was a novel satellite component of BdPV1/SXD111. BdHV1/SXD111 could be transmitted through conidia and hyphae contact, whereas it likely has no apparent impact on the morphologies and virulence of the host fungus. Thus, this study is the first report of a betahypovirus isolated from the fungus B. dothidea. Importantly, our results significantly enhance the diversity of the B. dothidea viruses.

Keywords: Betahypovirus; Botryosphaeria dothidea; Botryosphaeria dothidea hypovirus 1; Hypoviridae; genome.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Ascomycota*
Fungal Viruses*
Genome, Viral
Phylogeny
Polyproteins / genetics
RNA Viruses*
RNA, Double-Stranded / genetics
Viral Proteins / genetics

Substances

Viral Proteins
RNA, Double-Stranded
Polyproteins

Supplementary concepts

Botryosphaeria dothidea

Grants and funding

This work was financially supported by the National Natural Science Foundation of China (32072476); the Science and Technology Research Program of Chongqing Municipal Education Commission (KJQN202001411, KJQN202201401); the Innovation Research and Entrepreneurship Training Program for Undergraduate of Chongqing (S202210647031); and the Research Funding Project of Yangtze Normal University (2017KYQD54).