Manipulating the wavefront of coherent light incident on scattering media to enhance the imaging depth, sensitivity, and resolution is a common technique in biomedical applications. Local phase variations cause changes in the interference and can be used to create a focus inside or behind a scattering medium. We use wavefront shaping (WFS) to force constructive interference at an arbitrary location. The amount of light transmitted into a given region strongly depends on the scattering and absorption characteristics. These are described by their respective coefficients μs and μa and the scattering phase function. Controlling the scattering and absorption coefficients, we study the behavior of wavefront shaping and the achievable intensity enhancement behind volume scattering media with well-defined optical properties. The phantoms designed in this publication are made of epoxy resin. Into these epoxy matrices, specific amounts of scattering and absorbing particles, such as titanium dioxide pigments and molecular dyes, are mixed. The mixture obtained is filled into 3D-printed frames of various thicknesses. After a precise fabrication procedure, an integrating sphere-based setup characterizes the phantoms experimentally. It detects the total hemispherical transmission and reflection. Further theoretical characterization is performed with a newly developed hybrid PN method. This method senses the flux of light into a particular angular range at the lower boundary of a slab. The calculations are performed without suffering from ringing and fulfill the exact boundary conditions there. A decoupled two-path detection system allows for fast optimization as well as sensitive detection. The measurements yield results that agree well with the theoretically expected behavior.
Keywords: absorption coefficient; hybrid PN method; phantom; scattering coefficient; volume scattering; wavefront shaping.